FastQCFastQC Report
Sat 26 May 2018
H2JY7AFXY_n01_ds395-041-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2JY7AFXY_n01_ds395-041-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9564164
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATG3208663.3548776453435973TruSeq Adapter, Index 10 (100% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC96390.10078246253410125TruSeq Adapter, Index 10 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAGG447450.053.6449170
AAAAAAG526650.046.04964469
GCTTGAA596750.040.36665360
CTTGAAA603550.040.2649961
ATGCCGG65050.039.9776370
TGCTTGA593850.039.75627559
TGAAAAA635600.039.7153863
GAAAAAA641200.039.47769564
TTGAAAA620500.039.35103262
CTGCTTG600550.039.0209158
GACGTGT115600.038.589271
TCTGCTT640950.036.70881357
TTCTGCT643600.035.79627256
CTTCTGC655200.035.65381255
GTCTTCT657050.035.26373353
TCTTCTG685600.033.95037554
CCGTCTT710200.033.44274551
CGTCTTC708550.033.2835252
GCCGTCT734650.032.0865950
TGCCGTC772100.031.0290649
Produced by FastQC (version 0.11.5)