FastQCFastQC Report
Sat 26 May 2018
H2JY7AFXY_n01_ds395-041-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2JY7AFXY_n01_ds395-041-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9445783
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATG3658363.8730087278100713TruSeq Adapter, Index 5 (100% over 49bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAGG505800.054.34814570
AAAAAAG590350.046.93815669
ATGCCGG54300.046.4093370
GACGTGT89450.043.0189861
CTTGAAA725400.038.86620761
GCTTGAA738500.037.9871960
TGAAAAA756550.037.9365763
GAAAAAA769000.037.4498264
TTGAAAA756100.037.41310562
TGCTTGA751050.036.87241459
CTGCTTG763050.036.19621758
TCTGCTT812800.034.11833257
TTCTGCT818700.033.4235656
CTTCTGC826550.033.3305655
GTCTTCT849850.032.2676353
TCTTCTG860200.031.98195354
CCGTCTT906700.030.5957351
CGTCTTC906950.030.47152152
GCCGTCT939000.029.41639750
TGCCGTC969950.028.67982949
Produced by FastQC (version 0.11.5)