FastQCFastQC Report
Sat 26 May 2018
H2JY7AFXY_n01_ds395-032-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2JY7AFXY_n01_ds395-032-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11342631
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATG3618393.1900799735087917TruSeq Adapter, Index 11 (100% over 49bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAGG484600.054.19815470
AAAAAAG548400.048.35235669
ATGCCGG49300.046.7857570
GACGTGT112850.040.735791
GAAAAAA741800.038.00226264
CTTGAAA741800.037.7333161
TGAAAAA747800.037.64586663
GCTTGAA741850.037.5469360
TTGAAAA752050.037.24695662
TGCTTGA750850.036.82200659
CTGCTTG764450.036.1621858
TCTGCTT803350.034.44162457
TTCTGCT816900.033.77179356
CTTCTGC825250.033.53582455
GTCTTCT843300.032.84234653
TCTTCTG860100.032.1729354
ACGTGTG200800.030.8095821
CCGTCTT952200.029.09362451
CGTCTTC956550.028.9979152
GCCGTCT1018750.027.16547450
Produced by FastQC (version 0.11.5)