FastQCFastQC Report
Sat 26 May 2018
H2JY7AFXY_n01_ds395-032-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2JY7AFXY_n01_ds395-032-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10676523
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATG5249024.916413330444752TruSeq Adapter, Index 6 (100% over 49bp)
GGAAAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAA109660.10271134151071469TruSeq Adapter, Index 6 (100% over 38bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAGG664800.055.5329970
ATGCCGG38500.049.09134770
AAAAAAG776650.047.90939769
GACGTGT84100.041.4200741
CTTGAAA969050.040.084761
TGAAAAA985000.039.7909563
GAAAAAA1003350.039.2899764
TTGAAAA994650.039.16913262
GCTTGAA1012950.038.2025360
TGCTTGA1040150.037.03528259
CTGCTTG1069800.035.96629758
TCTGCTT1123900.034.29387757
CTTCTGC1150700.033.59858355
TTCTGCT1146600.033.59356756
GTCTTCT1194600.032.38979353
TCTTCTG1199350.032.2240354
ACGTGTG152700.030.8137131
CCGTCTT1270700.030.4856951
CGTCTTC1279050.030.2948852
GCCGTCT1329950.029.07213450
Produced by FastQC (version 0.11.5)