FastQCFastQC Report
Sat 26 May 2018
H2JY7AFXY_n01_787.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2JY7AFXY_n01_787.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6958854
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG279360.401445410408093TruSeq Adapter, Index 4 (100% over 49bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCTTG159500.029.05347864
TGCTTGA161700.028.63634165
GCTTGAA170500.027.26097566
CCGTCTT180550.025.66618557
TGCCGTC185050.025.09878255
GCCGTCT186150.024.93166556
CGTCTTC189000.024.64830458
CTTGAAA197350.023.97768467
CTTCTGC195650.023.72108361
ATGCCGT197550.023.56380554
GACGTGT29050.023.3817061
TCTGCTT201400.023.25238263
GTCTTCT203900.022.93295759
TATGCCG205100.022.71312953
TCTTCTG204750.022.68390760
ACGTGTG41950.021.8670391
GTATGCC216300.021.52086352
TTCTGCT220250.021.27822762
ATGCCGG8350.020.95791670
TTGAAAA240900.019.84639468
Produced by FastQC (version 0.11.5)