FastQCFastQC Report
Sat 26 May 2018
H2JY7AFXY_n01_786.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2JY7AFXY_n01_786.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4983656
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATG188560.37835677261833484TruSeq Adapter, Index 3 (100% over 49bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCTTG94750.026.85494264
TGCTTGA96700.026.31313765
GCTTGAA104150.024.83418366
GACGTGT16750.024.4549331
CCGTCTT107300.023.94251357
TGCCGTC107900.023.84181455
GCCGTCT108250.023.63539556
CGTCTTC112100.022.97976758
ATGCCGT113700.022.74874154
TATGCCG116600.022.30279553
GTATGCC121300.021.46748552
CTTCTGC120100.021.41969961
TCTGCTT123200.020.99436663
GTCTTCT127200.020.52698159
TCTTCTG127950.020.07840260
CTTGAAA130300.020.03821867
ACGTGTG21450.019.9060062
CGTATGC130200.019.78499251
CATCTCG128250.019.4033646
TTCTGCT136250.018.95784262
Produced by FastQC (version 0.11.5)