FastQCFastQC Report
Sat 26 May 2018
H2JY7AFXY_n01_776.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2JY7AFXY_n01_776.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7138858
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATG156880.21975503644980754TruSeq Adapter, Index 2 (100% over 49bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCTTG92250.025.68648164
TGCTTGA98550.024.29286865
CCGTCTT108950.022.4563157
TGCCGTC108900.022.4021855
GCCGTCT110050.022.32726356
GACGTGT26650.021.9399431
ATGCCGT115250.021.19824254
TATGCCG116950.021.0991653
GCTTGAA115700.020.99449366
CGTCTTC119650.020.62361758
GTATGCC126600.019.51853852
TCTGCTT127750.019.04172363
ACGTGTG32050.018.5641842
CTTCTGC131700.018.55047661
GTCTTCT133800.018.46872959
CGTATGC136700.018.10202451
TCTTCTG142050.017.4700260
TATCTCG139750.017.1558446
CTCGTAT141900.016.9454749
CTTGAAA150400.016.61612367
Produced by FastQC (version 0.11.5)