FastQCFastQC Report
Sat 26 May 2018
H2JY7AFXY_n01_163A.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2JY7AFXY_n01_163A.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6498983
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG406940.6261595083415359TruSeq Adapter, Index 1 (100% over 49bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTGT48850.026.3744471
GCTTGAA216650.025.6711366
TGCTTGA224500.024.55523165
CTTGAAA233950.023.92242467
CTGCTTG234100.023.54844964
AAAAAGG131200.023.2357970
TCTGCTT269800.020.61413463
ACGTGTG64300.020.4655422
TGAAAAA283250.020.17883569
TTGAAAA283750.019.92124468
CTTCTGC282900.019.62230561
TTCTGCT287550.019.26862362
CCGTCTT292400.019.0687257
GTGACTG18100.018.956041
GCCGTCT296300.018.73504356
GTCTTCT299500.018.65187659
CGTCTTC300450.018.6278558
GAAAAAA312500.018.48021570
TGCCGTC302800.018.29819355
TCTTCTG304600.018.22467460
Produced by FastQC (version 0.11.5)