FastQCFastQC Report
Thu 19 Jul 2018
000000000-BDGGG_l01_n01_Trinidad5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BDGGG_l01_n01_Trinidad5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2168859
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGTGC5550.015.8108118
TAGACTG3700.015.20270255
CTATACT7550.014.9006624
GTATTGG8600.014.3895351
GTCCTGG4900.014.2346941
GACTGCG2254.9039954E-914.07
GTACAGG5000.013.9500011
GTATTAG5500.013.9090911
TACACAG6350.013.8188995
GTATAGG6750.013.6666671
CCAGGAC4800.013.5937493
CCTATAC5800.013.5775873
CTACACA7650.013.5294114
TGTACTG8700.013.4482765
ACCGTGC7400.013.3783788
CTTACAC3201.8189894E-1213.3593753
GCTACAC6600.013.2954553
CTAGGAC5500.013.0909093
GGACCGT3101.6370905E-1113.0645166
TCTATAC6050.013.0165293
Produced by FastQC (version 0.11.5)