FastQCFastQC Report
Tue 22 May 2018
000000000-BDGGG_l01_Trinidad9_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BDGGG_l01_Trinidad9_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2582400
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACTG4650.016.9354845
GTATAGG8100.015.2777771
GTGTAAG4600.014.6739141
GACGTTA2152.586603E-914.65116220
GTATTGG11200.013.8616071
ATTCGCG5550.013.78378437
TATACTG9400.013.4042555
CTATACT11250.013.44
GTCCTAG5950.013.2352941
CCTAGAC4500.013.03
GTCCTGG5900.012.9661021
GTAGCAC7350.012.8571423
ACACTGT8600.012.8197676
TACACTG7050.012.7659575
ACGTTAG2502.1482265E-812.59999921
TATACAG10050.012.5373135
GTGTAGG7900.012.5316461
CTAGACT5950.012.47899154
AACACTG5450.012.3853215
TTAGACG3501.0913936E-1112.21428638
Produced by FastQC (version 0.11.5)