FastQCFastQC Report
Tue 22 May 2018
000000000-BDGGG_l01_Trinidad5_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BDGGG_l01_Trinidad5_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2291815
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTCGCG3600.015.037
CCAGGAC4600.014.6739143
TATACTG8650.013.7861275
GTGTATG9500.013.7368421
GTATTGG8850.013.7288131
GTATAGG7400.013.6824331
CCTATAC6450.013.60465053
GTCTAGG3850.013.4415591
GTGTAGG6800.013.2352931
CTGTCCG2959.640644E-1112.9661029
TACACTG7900.012.8164565
GACTGTG3355.456968E-1212.7611937
AGACAGT4250.012.7058826
ATACTGT8000.012.656256
TATTGGG9650.012.5906732
ACTGTGC7000.012.5357148
ACAGTGC6550.012.3664128
CAGGACC4750.012.3157894
CGTTCGT7050.012.12765911
TAACACG2452.2424138E-711.9387764
Produced by FastQC (version 0.11.5)