FastQCFastQC Report
Tue 22 May 2018
000000000-BDGGG_l01_Trinidad5_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BDGGG_l01_Trinidad5_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2291815
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACTG4700.015.3193435
CCAGGAC5950.013.9917743
GTCCTAG5450.013.6237281
ATTCGCG3800.013.61829637
GTATAAG7000.013.4998761
CCTATAC7200.013.1251653
GTATTGG9350.012.9945331
ATACTGT8350.012.9342966
GTAGGAC4700.012.9256953
CAGGACC6800.012.9045734
TACACTG6350.012.7560675
TAGGACT5500.012.6819784
TATACAG8400.012.5894455
AGACTGT3800.012.4343676
GTGTTAC4550.012.3625231
CTACACA9300.012.3388654
GTGTAGG6750.012.333221
GTGTAAG3651.8189894E-1212.3286531
CCCAGGG4200.012.3213152
TACACGG3850.012.2728825
Produced by FastQC (version 0.11.5)