FastQCFastQC Report
Tue 22 May 2018
000000000-BDGGG_l01_Trinidad4_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BDGGG_l01_Trinidad4_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2366291
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTGG7900.017.3734191
CCTACAC6600.017.0454563
TATACTG9250.015.081085
ATTCGCG4300.013.60465137
CTATACT9850.013.0203064
CCGTGCG5900.012.9661029
TACACCG4550.012.8571425
GTGTATG8000.012.656251
TAGACTG4450.012.640455
GGACCGT2351.3040335E-712.4468096
ACTGTCC3850.012.2727278
TCTATAC7000.012.2142863
ACAGTCC3701.8189894E-1212.1621638
GTCTTAG3701.8189894E-1212.1621631
GTCTAGG3900.012.1153841
GTGCTAG6750.012.0000011
CCTATAC7700.011.9805193
CTACACC6950.011.9784174
GTATAGG7950.011.8867921
ACCGTGC6850.011.8248178
Produced by FastQC (version 0.11.5)