FastQCFastQC Report
Tue 22 May 2018
000000000-BDGGG_l01_Trinidad4_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BDGGG_l01_Trinidad4_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2366291
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACTG4550.018.791515
GTATAGG7750.015.3873421
GTATTGG9350.015.1606691
ACTGTGC5650.015.1329858
GTGTAGG6700.015.1121821
CCAGGAC5550.015.000243
GTCCTAG4150.014.6387871
CTATACT10400.014.0627244
TATACTG8650.014.0464675
GTGTAAG4700.013.8832011
CTGTCTG3950.013.67110359
CTTACAC4600.013.2067323
CTAGGAC4700.012.9257383
GCTAGAC3355.456968E-1212.7613973
TATTGGG9350.012.7542132
GTCCAGG5650.012.74356651
TACACTG7300.012.6371885
AGACCGT3051.6916601E-1012.5411836
TGCGTTC6300.012.5001999
GTGTATG10350.012.3915021
Produced by FastQC (version 0.11.5)