FastQCFastQC Report
Tue 22 May 2018
000000000-BDGGG_l01_Trinidad1_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BDGGG_l01_Trinidad1_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2567466
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG9600.015.9375015
GTCTAAG5150.014.8543681
CCTACAC6500.014.1923083
TAGACAG4700.013.8829785
TCTATAC7200.013.753
GTAAGAC3300.013.6363633
GTATAGG7600.013.6184211
ACTGTGC6350.013.4645678
ACCGTGC7600.013.3223698
GTGTACG4600.013.2065231
TAGACTG4800.013.1250015
TTATACT10550.013.00947954
CTATACT10300.012.888354
GTATAGA7450.012.6845651
CTACACC8900.012.640454
GTCTATG6300.012.51
CTAGACA6500.012.4615384
GTGTAGG7600.012.4342111
TACACTG7450.012.3825515
GTCCTAG6000.012.3751
Produced by FastQC (version 0.11.5)