FastQCFastQC Report
Wed 20 Dec 2017
HYTFNBCXY_l02n02_sambamu12112017-4.352000000ad47f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYTFNBCXY_l02n02_sambamu12112017-4.352000000ad47f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35104049
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGATCATTTCTTCGGAAGATA1658470.47244407618049983No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTAAATTTTATTAAGGTTTC950360.2707266047856759No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACCAGTCTTCTACAGGACA805570.22948065050843566No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGATCTGTTTTCTACAGCATT762840.21730826549381813No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGAATTAATTATTTTTGTA632850.180278349087309No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATCATTTCTTCGGAAGATAC519170.147894620361315No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATCTGTTTTCTACAGCATTA482600.13747701867667744No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACCAGTCTTCTACAGGACAC460670.13122987607497927No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT2564500.086.004211
AGCAGTG2724200.080.814112
GCAGTGG2985650.073.673673
GGTATCA3001200.073.490588
TGGTATC3020900.072.803597
CAGTGGT3025900.072.684174
GTGGTAT3051550.072.107676
GTATCAA3074400.071.8984769
AGTGGTA3093800.071.230525
AGAGTAC2957000.039.62075818-19
GCAGAGT2972650.039.45640616-17
CAACGCA3003150.039.1974312-13
TACATGG2980150.038.9694822-23
CATGGGG2133200.038.53226524-25
ACGCAGA3084700.038.12177314-15
ATCAACG3139400.037.5783910-11
AGTACAT3124450.037.4797320-21
CGCAGAG2940250.037.47149316-17
CAGAGTA2995150.036.81123418-19
GAGTACA3006850.036.66549720-21
Produced by FastQC (version 0.11.2)