FastQCFastQC Report
Wed 20 Dec 2017
HYTFNBCXY_l02n02_sambamu12112017-3.352000000ad456.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYTFNBCXY_l02n02_sambamu12112017-3.352000000ad456.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28151957
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGATCATTTCTTCGGAAGATA1236500.43922346144532687No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTAAATTTTATTAAGGTTTC717370.2548206506567199No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACCAGTCTTCTACAGGACA607000.2156155609359591No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGATCTGTTTTCTACAGCATT568920.20208897022683003No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGAATTAATTATTTTTGTA480610.1707199254389313No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATCATTTCTTCGGAAGATAC381240.13542220173183697No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATCTGTTTTCTACAGCATTA363540.12913489460075547No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACCAGTCTTCTACAGGACAC348400.1237569381055818No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1939300.085.223891
AGCAGTG2042700.080.729032
GGTATCA2249950.073.424218
GCAGTGG2249750.073.2743
CAGTGGT2278400.072.347434
TGGTATC2283700.072.167657
GTATCAA2297000.072.125869
GTGGTAT2294300.071.904426
AGTGGTA2329200.070.93325
AGAGTAC2215400.039.47043218-19
GCAGAGT2234150.039.15761616-17
TACATGG2225000.038.91583622-23
CAACGCA2258550.038.91314712-13
CATGGGG1603800.038.5191924-25
ATCAACG2345950.037.5500310-11
CGCAGAG2197700.037.5368516-17
AGTACAT2333800.037.39446320-21
ACGCAGA2351950.037.3383614-15
CAGAGTA2239000.036.86420418-19
GAGTACA2251450.036.6748620-21
Produced by FastQC (version 0.11.2)