FastQCFastQC Report
Wed 20 Dec 2017
HYTFNBCXY_l02n02_sambamu12112017-2.352000000ad43c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYTFNBCXY_l02n02_sambamu12112017-2.352000000ad43c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52449082
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGATCATTTCTTCGGAAGATA2598080.49535280712825436No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTAAATTTTATTAAGGTTTC1422860.2712840617496413No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACCAGTCTTCTACAGGACA1265970.24137124077786531No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGATCTGTTTTCTACAGCATT1169250.22293049857383587No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGAATTAATTATTTTTGTA933740.17802790142256447No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATCATTTCTTCGGAAGATAC804790.1534421517615885No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATCTGTTTTCTACAGCATTA739510.1409957947405066No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACCAGTCTTCTACAGGACAC720720.13741327255260635No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT3897400.086.048541
AGCAGTG4158400.080.49792
GCAGTGG4568200.073.246513
GGTATCA4580900.073.201858
TGGTATC4612900.072.486147
CAGTGGT4619000.072.41674
GTGGTAT4650550.071.981036
GTATCAA4679550.071.795249
AGTGGTA4716800.071.028425
AGAGTAC4517400.039.40688318-19
GCAGAGT4534850.039.28860516-17
CAACGCA4574950.039.11036312-13
TACATGG4537950.038.86093522-23
CATGGGG3259300.038.41752624-25
ACGCAGA4714000.037.9136114-15
AGTACAT4744750.037.4808520-21
ATCAACG4794450.037.43998710-11
CGCAGAG4493700.037.24711216-17
CAGAGTA4568100.036.6649918-19
GAGTACA4593950.036.45376220-21
Produced by FastQC (version 0.11.2)