FastQCFastQC Report
Wed 20 Dec 2017
HYTFNBCXY_l02n02_sambamu12112017-1.352000000ad412.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYTFNBCXY_l02n02_sambamu12112017-1.352000000ad412.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33607796
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGATCATTTCTTCGGAAGATA1563530.46522836546615554No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTAAATTTTATTAAGGTTTC865680.25758309173264443No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACCAGTCTTCTACAGGACA747520.22242458267718598No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGATCTGTTTTCTACAGCATT702600.20905863627594026No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGAATTAATTATTTTTGTA576980.17168040415384572No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATCATTTCTTCGGAAGATAC484700.14422248932955914No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATCTGTTTTCTACAGCATTA443890.1320794734650258No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACCAGTCTTCTACAGGACAC421270.12534889226297374No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT2346300.085.388011
AGCAGTG2486200.080.482912
GGTATCA2738800.073.200988
GCAGTGG2743250.072.892963
TGGTATC2775100.072.063677
CAGTGGT2774850.072.0565264
GTATCAA2793750.071.8919
GTGGTAT2792950.071.6422656
AGTGGTA2832750.070.7212145
AGAGTAC2696150.039.47049718-19
GCAGAGT2715600.039.2349216-17
CAACGCA2746900.038.95345712-13
TACATGG2707000.038.9314522-23
CATGGGG1941500.038.63584524-25
AGTACAT2829050.037.56743620-21
ACGCAGA2852600.037.51898614-15
ATCAACG2861600.037.4998410-11
CGCAGAG2678050.037.35279516-17
CAGAGTA2726550.036.70668818-19
GAGTACA2733550.036.57777820-21
Produced by FastQC (version 0.11.2)