FastQCFastQC Report
Wed 20 Dec 2017
HYTFNBCXY_l02n01_sambamu12112017-4.351000000ad472.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYTFNBCXY_l02n01_sambamu12112017-4.351000000ad472.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35104049
Sequences flagged as poor quality0
Sequence length101
%GC23

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACTAG102300.078.6662141
GACTAAC83350.074.081621
CCGTACT138850.073.911771
GTACTCC124200.073.67431
GGGACCT124600.073.285191
CGTCTAC103500.073.2073751
GTTCTCG117450.072.970881
CGCTATC94650.072.5694961
TGACTAC82900.072.366899
GGCTCGA115700.072.266891
CGTGAGC79450.071.9146651
GACGTGC101050.071.6424561
CGAGCAC163350.070.712141
GGGTTGC121750.070.666981
AGGTCCG122500.070.311921
TGGCCAG146200.069.8383261
CCGGTAG82250.069.177541
GGGAATG147850.069.123231
CTAAGAC87000.068.788111
ACGAGCC94000.068.62131
Produced by FastQC (version 0.11.2)