FastQCFastQC Report
Wed 20 Dec 2017
HYTFNBCXY_l02n01_sambamu12112017-3.351000000ad459.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYTFNBCXY_l02n01_sambamu12112017-3.351000000ad459.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28151957
Sequences flagged as poor quality0
Sequence length101
%GC24

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACTAG77950.076.7753751
CGTCTAC79250.074.736211
GGCTCGA88100.074.2968441
CCGTACT97300.074.1114041
GACTAAC59750.074.066891
GTACTCC92900.073.783941
CGCTATC69300.073.737351
CGTGAGC59350.072.643851
TGGCCAG111450.071.483581
GTTCTCG92000.070.940631
GGGAATG110250.070.79571
GGGACCT92850.070.751961
TGACTAC61800.070.425259
GGGTTGC91500.069.925621
AGGTCCG94150.069.8760151
ACAGCCG87300.069.859391
GACGTGC75250.069.3598561
CCTCTGA68950.069.285781
CCTTACG62200.069.086051
CCACTAC84150.068.859191
Produced by FastQC (version 0.11.2)