FastQCFastQC Report
Wed 20 Dec 2017
HYTFNBCXY_l02n01_sambamu12112017-2.351000000ad43f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYTFNBCXY_l02n01_sambamu12112017-2.351000000ad43f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52449082
Sequences flagged as poor quality0
Sequence length101
%GC23

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACTAG155800.077.066741
GTACTCC180850.074.093081
CGTCTAC155800.073.558171
CCGTACT202300.073.403411
GACTAAC121050.072.8811
CGTGAGC118150.072.698521
GGCTCGA172050.072.633511
GACGTGC146600.072.0137941
GTTCTCG176250.071.7926561
CGCTATC141750.071.694481
GGGACCT184750.071.191121
TGACTAC119400.070.556899
ACGAGCC139550.070.3041461
CGAGCAC255100.070.061351
ACAGCCG178350.069.801291
TGGCCAG221700.069.746031
CTCGAGG172650.069.435181
GGGTTGC184600.069.1632461
GTAACTG179700.068.800781
TGCGTGG166750.068.670791
Produced by FastQC (version 0.11.2)