FastQCFastQC Report
Wed 20 Dec 2017
HYTFNBCXY_l02n01_sambamu12112017-1.351000000ad415.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYTFNBCXY_l02n01_sambamu12112017-1.351000000ad415.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33607796
Sequences flagged as poor quality0
Sequence length101
%GC24

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACTAG93050.077.0393751
CGTCTAC96300.075.6241151
CGCTATC85350.074.521291
CGTGAGC70050.074.375561
CCGTACT120700.073.963271
GACTAAC76400.073.918111
GTACTCC115900.073.1710361
GACGTGC88100.071.547651
GTTCTCG107400.071.349111
GGCTCGA110600.071.046971
TGACTAC74850.070.893299
GGGACCT113000.070.884171
GGGAATG135850.070.508781
ACGAGCC86050.069.9375841
CCTCTGA80350.069.8110051
CTCGAGG102700.069.754241
CCACTAC96650.069.5956951
TGCGTGG103200.069.370221
TGGCCAG131700.069.30161
CGTTAGA96550.069.126191
Produced by FastQC (version 0.11.2)