FastQCFastQC Report
Wed 20 Dec 2017
HYTFNBCXY_l01n02_whole_embryo_12hpf_4.352000000ad462.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYTFNBCXY_l01n02_whole_embryo_12hpf_4.352000000ad462.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29584358
Sequences flagged as poor quality0
Sequence length101
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGTAAATTTTATTAAGGTTTC1198530.4051228693216868No Hit
GCAGTGGTATCAACGCAGAGTACATGGGGTAAATTTTATTAAGGTTTCTT431390.14581692122573692No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT886150.073.7695161
AGCAGTG1025900.063.4271132
GCAGTGG1317050.049.3088343
CAGTGGT1336800.048.498714
AGTGGTA1364200.047.5537535
TGGTATC1413600.045.739117
GTGGTAT1459850.044.3827066
GGTATCA1464900.044.21848
GTATCAA1543800.042.164629
AGAGTAC1460300.025.33500718-19
ACGCAGA1511800.024.7741214-15
GCAGAGT1498950.024.7666516-17
TACATGG1509100.024.06674222-23
CAACGCA1566550.023.98081812-13
AGTACAT1564000.023.55678720-21
CATGGGG878100.023.53719124-25
ATCAACG1633100.023.054310-11
CGCAGAG1446450.022.40176416-17
AACGCAG1504250.021.66223314-15
CAGAGTA1490700.021.65770718-19
Produced by FastQC (version 0.11.2)