FastQCFastQC Report
Wed 20 Dec 2017
HYTFNBCXY_l01n02_whole_embryo_12hpf_3.352000000ad449.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYTFNBCXY_l01n02_whole_embryo_12hpf_3.352000000ad449.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24959434
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGTAAATTTTATTAAGGTTTC988830.39617484915723644No Hit
GCAGTGGTATCAACGCAGAGTACATGGGGTAAATTTTATTAAGGTTTCTT349730.14011936328363858No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT738350.074.143641
AGCAGTG855800.063.71782
GCAGTGG1095100.049.738683
CAGTGGT1120150.048.6055644
AGTGGTA1144450.047.583265
TGGTATC1184300.045.900377
GGTATCA1221050.044.546138
GTGGTAT1224550.044.4280856
GTATCAA1283900.042.580049
AGAGTAC1210900.025.49727218-19
GCAGAGT1243950.024.91077816-17
ACGCAGA1267400.024.67246614-15
TACATGG1248800.024.33981322-23
CAACGCA1303200.024.0745812-13
AGTACAT1296100.023.73679420-21
CATGGGG727300.023.64629224-25
ATCAACG1359050.023.15126210-11
CGCAGAG1203500.022.61660216-17
CAGAGTA1236450.021.91079718-19
AACGCAG1257950.021.7279314-15
Produced by FastQC (version 0.11.2)