FastQCFastQC Report
Wed 20 Dec 2017
HYTFNBCXY_l01n02_whole_embryo_12hpf_2.352000000ad42f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYTFNBCXY_l01n02_whole_embryo_12hpf_2.352000000ad42f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25959242
Sequences flagged as poor quality0
Sequence length101
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGTAAATTTTATTAAGGTTTC1051270.4049694517274426No Hit
GCAGTGGTATCAACGCAGAGTACATGGGGTAAATTTTATTAAGGTTTCTT379170.14606358691058852No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT767050.074.336691
AGCAGTG891300.063.5146872
GCAGTGG1143650.049.41733
CAGTGGT1161250.048.6191564
AGTGGTA1193200.047.3710565
TGGTATC1237500.045.538477
GTGGTAT1275700.044.2777676
GGTATCA1284250.043.9399228
GTATCAA1355200.041.954899
AGAGTAC1283050.025.16051118-19
ACGCAGA1329300.024.64073214-15
GCAGAGT1312350.024.63934116-17
TACATGG1319250.024.019722-23
CAACGCA1368650.024.01188912-13
AGTACAT1365250.023.55910520-21
CATGGGG775200.023.4418124-25
ATCAACG1432850.023.03414710-11
CGCAGAG1267450.022.24659216-17
CAGAGTA1304150.021.4630518-19
AACGCAG1323600.021.39380314-15
Produced by FastQC (version 0.11.2)