FastQCFastQC Report
Wed 20 Dec 2017
HYTFNBCXY_l01n02_whole_embryo_12hpf_1.352000000ad405.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYTFNBCXY_l01n02_whole_embryo_12hpf_1.352000000ad405.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30918173
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGTAAATTTTATTAAGGTTTC1270490.4109201407211222No Hit
GCAGTGGTATCAACGCAGAGTACATGGGGTAAATTTTATTAAGGTTTCTT451930.1461696976726277No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT932850.073.980971
AGCAGTG1083800.063.377892
GCAGTGG1378750.049.6447833
CAGTGGT1393900.049.0744634
AGTGGTA1429350.047.9180875
TGGTATC1496850.045.61267
GTGGTAT1543750.044.2687266
GGTATCA1548600.044.115958
GTATCAA1627050.042.213619
AGAGTAC1537900.025.35838318-19
ACGCAGA1593650.024.75747314-15
GCAGAGT1579250.024.75508516-17
TACATGG1588300.024.08999322-23
CAACGCA1652000.023.93597212-13
AGTACAT1642000.023.66714120-21
CATGGGG929700.023.39867224-25
ATCAACG1723800.023.04783210-11
CGCAGAG1526500.022.3390116-17
CAGAGTA1571350.021.59949918-19
AACGCAG1587050.021.55967314-15
Produced by FastQC (version 0.11.2)