FastQCFastQC Report
Wed 20 Dec 2017
HYTFNBCXY_l01n02_sambamu12112017-index.352000000ad48c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYTFNBCXY_l01n02_sambamu12112017-index.352000000ad48c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8165430
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACTGCGACCTCCCTAGCAAACTGGGGCACAAACTTAATTGAACCCATG722102388.43408124250652No Hit
ATACTGCGACCTCCCTAGCAAACCATGCCACCTATGTAAGGTTGCTGGGT268670.3290334985420241No Hit
NTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN243980.29879626669997783No Hit
ATACTGCGACCTCCCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA204280.25017665940434247Illumina Single End PCR Primer 1 (100% over 34bp)
ATACTGCGACCTCCCTAGCAAACTGGGGCACAAACTTAATTGACTTTGGG158570.19419675387579097No Hit
ATACTGCGACCTCCCTAGCAAACTGGGGCACAAACTTAATTGATATCCCG147630.18079880667643958No Hit
ATACTGCGACCTCCCTAGCAAGGTGGTTATTGTAAAGCTGAAGCTTGACA117790.14425449731367485No Hit
ATACTGCGACCTCCCTAGCAAACTGGGACACAAACTTAATTGAACCCATG113670.13920883529710007No Hit
ATACTGCGACCTCCCTAGCAAACTGGGGCACAATAGGTGGACTGATCGTC101790.12465969336581172No Hit
ATACTGTGACCTCCCTAGCAAACTGGGGCACAAACTTAATTGAACCCATG89570.10969416185063124No Hit
ATACTGCGACCTCTCTAGCAAACTGGGGCACAAACTTAATTGAACCCATG83860.10270126619173761No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGACCT3200.094.995666
TGGGACC3200.094.995085
TTCGAAC156.620749E-494.995085
GACCTAG600.094.989248
TAATGCG409.094947E-1294.9892352
ATACTGC7819400.094.93031
TACTGCG7810200.094.86582
ACTGCGA7814500.094.816583
CTGCGAC7804700.094.7895364
TGCGACC7801950.094.7448655
GCGACCT7803800.094.719336
CGACCTC7808550.094.683297
GACCTCC7808650.094.611538
ACCTCCC7809500.094.578129
CGATCTC8900.094.455597
GAGACCT6650.094.28146
GATCTCC9050.093.939638
TACTACG10500.093.632252
TGAGACC6700.093.577245
TGCGATC9350.093.4710855
Produced by FastQC (version 0.11.2)