FastQCFastQC Report
Wed 20 Dec 2017
HYTFNBCXY_l01n01_whole_embryo_12hpf_4.351000000ad465.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYTFNBCXY_l01n01_whole_embryo_12hpf_4.351000000ad465.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29584358
Sequences flagged as poor quality0
Sequence length101
%GC20

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTATC90650.076.441591
GACTAAC74600.074.514391
TGACTAG80000.074.124911
CGTGAGC76950.073.6612851
GATCTAG83650.073.621471
CCTTACG83600.072.697721
GCGCGAT90150.072.378221
CTGCGGA82000.071.7365951
CCACTAC89300.071.46831
CTGCCTA88550.070.99871
GCTGCAG92000.070.7158661
CCGTACT82350.069.697921
CGCTTCA79450.069.606281
ACGAGCC83850.069.415991
GTCGACT60800.068.855828
GGATTAC99950.068.709961
CTGATCC81050.068.643221
CCTACCA79550.068.6213761
GACTGCG90600.068.0789641
GTACTCC85800.068.004761
Produced by FastQC (version 0.11.2)