FastQCFastQC Report
Wed 20 Dec 2017
HYTFNBCXY_l01n01_whole_embryo_12hpf_3.351000000ad44c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYTFNBCXY_l01n01_whole_embryo_12hpf_3.351000000ad44c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24959434
Sequences flagged as poor quality0
Sequence length101
%GC21

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACTAG69300.076.1602861
GACTAAC62600.074.504421
CGCTATC72400.074.3454361
CGCTTCA67250.072.749681
GCGCGAT74800.072.596271
CCTTACG74400.072.53881
CGTGAGC61900.072.425341
GATCTAG73800.071.451881
CTGCCTA78150.069.8497241
CCACTAC76250.069.655371
CTGATCC64750.069.605011
GGATTAC82650.069.156081
CCTACCA65200.068.6866461
CTGCGGA72350.068.673981
ACGAGCC72450.068.644881
TGCGTGG74600.068.5803761
CGTCTAC63100.068.5590441
GATCGCG65750.068.473991
CCGTACT71200.067.577391
CCGGTAG64950.067.5588151
Produced by FastQC (version 0.11.2)