FastQCFastQC Report
Wed 20 Dec 2017
HYTFNBCXY_l01n01_whole_embryo_12hpf_2.351000000ad422.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYTFNBCXY_l01n01_whole_embryo_12hpf_2.351000000ad422.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25959242
Sequences flagged as poor quality0
Sequence length101
%GC20

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACTAG73500.074.917461
GACTAAC66500.073.786011
CCTTACG70100.073.051861
CGCTATC73950.073.045711
CTGCCTA80550.072.319081
GCGCGAT78150.071.799571
CGTGAGC68400.071.38851
CTGCGGA75750.070.241881
CGCTTCA70400.070.239251
GATCGCG68800.070.143351
GATCTAG76650.069.727581
CCACTAC80150.069.711041
ACGAGCC76800.069.653361
CCTACCA71750.069.183011
TGCGTGG79300.069.017881
GGATTAC82150.068.419411
CCGTACT73800.068.357551
CCGGTAG69450.068.116311
CTCGAGG79000.068.075111
GCTGCAG77650.067.910251
Produced by FastQC (version 0.11.2)