FastQCFastQC Report
Tue 19 Dec 2017
HYTFNBCXY_l01n01_whole_embryo_12hpf_1.351000000ad408.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYTFNBCXY_l01n01_whole_embryo_12hpf_1.351000000ad408.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30918173
Sequences flagged as poor quality0
Sequence length101
%GC20

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTATC90950.075.933641
GACTAAC77050.073.633111
TGACTAG81750.073.533481
CCTTACG94000.073.216671
CTGCCTA92350.072.566351
GCGCGAT94500.072.2752461
GATCTAG91150.072.2684861
CCACTAC94200.071.899111
CGTGAGC82050.071.582371
CTGCGGA86350.071.049351
GGATTAC101600.070.691151
CGCTTCA83750.070.01571
CCTACCA87300.069.730991
TGCGTGG92500.069.721551
GATCGCG79400.069.235851
ACGAGCC95800.069.009081
CCGGTAG83350.068.867011
ACTGATG87950.068.025061
CTGATCC84900.067.441531
GCTGCAG94050.067.307331
Produced by FastQC (version 0.11.2)