FastQCFastQC Report
Tue 19 Dec 2017
HYTFNBCXY_l01n01_sambamu12112017-index.351000000ad48f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYTFNBCXY_l01n01_sambamu12112017-index.351000000ad48f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8165430
Sequences flagged as poor quality0
Sequence length101
%GC23

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGCAGAGTACGTTCCTCTGGAGAATTTTTTTTTTTTTTTTTTTTTTTT697051985.36622076240933No Hit
GTGCAGCAGTGCGATGGCCTGCCCGGTTATTATTATTTTTGACACCAGAC1682672.0607242974344278No Hit
AAGGCAGAGTACGTTCCTCTGGAGAATTTTTTTTTTTTTTTTTTTTTTTC1412921.730368149625923No Hit
AAGGCAGAGTACGTTCCTCTGGAGAATTTTTTTTTTTTTTTTTTTTTTCC744960.912334071812507No Hit
AAGGCAGAGTACGTTCCTCTGGAGAATTTTTTTTTTTTTTTTTTTTTCCA406420.49773251378065825No Hit
AAGGCAGAGTACGTTCCTCTGGAGAATTTTTTTTTTTTTTTTTTTTCCAA208810.2557244382720812No Hit
AGGGAGGTCGCAGTATCTGTCTCTTATACACATCTCCGAGCCCACGAGAC194290.23794215368939542No Hit
AAGGCAGAGTATGTTCCTCTGGAGAATTTTTTTTTTTTTTTTTTTTTTTT151070.18501168952523014No Hit
AAGGCAGAGTACGTTCCTCTGGAGATTTTTTTTTTTTTTTTTTTTTTTTT114200.13985791317787305No Hit
CGTGTCTCACAACGCCTCCCACTATATTTTTTTTTTTTTTTTTTTTTTTT102910.12603132964216213No Hit
AAGGCAGAGTACGTTCCTCTGGAGAATTTTTTTTTTTTTTTTTTTCCAAA102760.12584762835515093No Hit
AAGACAGAGTACGTTCCTCTGGAGAATTTTTTTTTTTTTTTTTTTTTTTT98160.12021412222014027No Hit
NAGGCAGAGTACGTTCCTCTGGAGAATTTTTTTTTTTTTTTTTTTTTTTT89840.11002482416725146No Hit
AAGGCAGAGTACGTTCCTCTGGAGAATTTTTTTTTTTTTTTTTTTTTTCA81810.10019068193591765No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGAAGG156.5640244E-495.200951
CGTTAGA156.5640244E-495.200951
CTACGTC156.5640244E-495.200951
GACCTGG156.5640244E-495.200951
GTGCAGC172500.095.200941
GATCGAT3900.095.200941
GACACGC700.095.200941
CGTGAGC254.5654087E-795.200941
CGCTATC254.5654087E-795.200941
ACAGCCG2700.095.200941
CGTGTAA550.095.200941
ACGAGCC700.095.200941
GGCTCGA254.5654087E-795.200941
TGACTAG2350.095.200941
AAGGCAG7446150.095.190081
GTGTCTC11900.095.078352
GGAGCTG750.095.078342
AGGAGCC700.095.078342
ACCGCGT1400.095.078342
CGCGAGG700.095.078342
Produced by FastQC (version 0.11.2)