FastQCFastQC Report
Thu 7 Apr 2022
HYNVTDRXY_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYNVTDRXY_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1122125197
Sequences flagged as poor quality0
Sequence length8
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCCTCCT12344671711.001153643999317No Hit
CGCGATAC1010130269.001939023386889No Hit
CAATGGAG981143398.743617847839843No Hit
TGGAATTA933102948.315497615548152No Hit
GTTGCAGC897638917.999454182116543No Hit
ACGCGGGT814547767.258973973471875No Hit
GAATTCCA737648426.5736730800814565Illumina Small RNA Adapter 2 (100% over 8bp)
TTTACATG625794545.576869155715073No Hit
GGGCTCTC550312464.904198403807877No Hit
TTCGCTGA494869424.410108794660637No Hit
CCGATTAA448560103.99741580707059No Hit
TTCGCCCT447491213.9878902211301117No Hit
GGATGGGC438303373.906011300448501No Hit
ACAAGAAT427640763.8109897286265104No Hit
AATCAATG357820033.1887710119747004No Hit
CATTAGCG339930343.0293441490201203No Hit
GGGGGGGG153637781.3691679004334842No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)