Loading report..

Highlight Samples

This report has flat image plots that won't be highlighted.
See the documentation for help.

Regex mode off

    Rename Samples

    This report has flat image plots that won't be renamed.
    See the documentation for help.

    Click here for bulk input.

    Paste two columns of a tab-delimited table here (eg. from Excel).

    First column should be the old name, second column the new name.

    Regex mode off

      Show / Hide Samples

      This report has flat image plots that won't be hidden.
      See the documentation for help.

      Regex mode off

        Export Plots

        px
        px
        X

        Download the raw data used to create the plots in this report below:

        Note that additional data was saved in multiqc_data when this report was generated.


        Choose Plots

        If you use plots from MultiQC in a publication or presentation, please cite:

        MultiQC: Summarize analysis results for multiple tools and samples in a single report
        Philip Ewels, Måns Magnusson, Sverker Lundin and Max Käller
        Bioinformatics (2016)
        doi: 10.1093/bioinformatics/btw354
        PMID: 27312411

        Save Settings

        You can save the toolbox settings for this report to the browser.


        Load Settings

        Choose a saved report profile from the dropdown box below:

        About MultiQC

        This report was generated using MultiQC, version 1.9

        You can see a YouTube video describing how to use MultiQC reports here: https://youtu.be/qPbIlO_KWN0

        For more information about MultiQC, including other videos and extensive documentation, please visit http://multiqc.info

        You can report bugs, suggest improvements and find the source code for MultiQC on GitHub: https://github.com/ewels/MultiQC

        MultiQC is published in Bioinformatics:

        MultiQC: Summarize analysis results for multiple tools and samples in a single report
        Philip Ewels, Måns Magnusson, Sverker Lundin and Max Käller
        Bioinformatics (2016)
        doi: 10.1093/bioinformatics/btw354
        PMID: 27312411

        A modular tool to aggregate results from bioinformatics analyses across many samples into a single report.

        Report generated on 2024-05-17, 16:26 based on data in: /vast/gencore/GENEFLOW/work/63/6b867052aef0f8b4575817946c3525/2


        General Statistics

        Showing 258/258 rows and 3/5 columns.
        Sample Name% Dups% GCM Seqs
        HYN72DRX3_l02_n01_AtbZIP16_An1AmpPoolHmB-B_aj1
        20.6%
        37%
        2.6
        HYN72DRX3_l02_n01_AtbZIP16_An1AmpPoolHmB-B_aj2
        20.9%
        37%
        2.6
        HYN72DRX3_l02_n01_AtbZIP16_C24AmpPoolHmB-B_aj1
        19.9%
        36%
        2.7
        HYN72DRX3_l02_n01_AtbZIP16_C24AmpPoolHmB-B_aj2
        20.5%
        37%
        2.8
        HYN72DRX3_l02_n01_AtbZIP16_ColAmpPoolHmB-B_a2
        22.2%
        37%
        3.5
        HYN72DRX3_l02_n01_AtbZIP16_ColAmpPoolHmB-B_aj1
        22.3%
        36%
        3.5
        HYN72DRX3_l02_n01_AtbZIP16_CviAmpPoolHmB-B_aj1
        21.2%
        36%
        2.7
        HYN72DRX3_l02_n01_AtbZIP16_CviAmpPoolHmB-B_aj2
        21.6%
        37%
        2.7
        HYN72DRX3_l02_n01_AtbZIP16_EriAmpPoolHmB-B_aj1
        16.8%
        36%
        2.4
        HYN72DRX3_l02_n01_AtbZIP16_EriAmpPoolHmB-B_aj2
        16.6%
        37%
        2.4
        HYN72DRX3_l02_n01_AtbZIP16_KyoAmpPoolHmB-B_aj1
        20.7%
        36%
        2.5
        HYN72DRX3_l02_n01_AtbZIP16_KyoAmpPoolHmB-B_aj2
        20.9%
        37%
        2.6
        HYN72DRX3_l02_n01_AtbZIP16_LerAmpPoolHmB-B_aj1
        21.7%
        36%
        2.6
        HYN72DRX3_l02_n01_AtbZIP16_LerAmpPoolHmB-B_aj2
        22.0%
        37%
        2.7
        HYN72DRX3_l02_n01_AtbZIP16_ShaAmpPoolHmB-B_aj1
        17.1%
        36%
        2.7
        HYN72DRX3_l02_n01_AtbZIP16_ShaAmpPoolHmB-B_aj2
        17.7%
        37%
        2.8
        HYN72DRX3_l02_n01_AtbZIP18_An1AmpPoolHmB-B_aj1
        23.6%
        37%
        3.6
        HYN72DRX3_l02_n01_AtbZIP18_An1AmpPoolHmB-B_aj2
        22.7%
        37%
        2.5
        HYN72DRX3_l02_n01_AtbZIP18_C24AmpPoolHmB-B_aj1
        21.9%
        36%
        3.0
        HYN72DRX3_l02_n01_AtbZIP18_C24AmpPoolHmB-B_aj2
        20.8%
        36%
        2.1
        HYN72DRX3_l02_n01_AtbZIP18_ColAmpPoolHmB-B_aj1
        24.0%
        37%
        3.7
        HYN72DRX3_l02_n01_AtbZIP18_ColAmpPoolHmB-B_aj2
        22.7%
        37%
        2.5
        HYN72DRX3_l02_n01_AtbZIP18_CviAmpPoolHmB-B_aj1
        22.7%
        37%
        3.0
        HYN72DRX3_l02_n01_AtbZIP18_CviAmpPoolHmB-B_aj2
        21.7%
        37%
        2.1
        HYN72DRX3_l02_n01_AtbZIP18_EriAmpPoolHmB-B_aj1
        18.6%
        36%
        2.9
        HYN72DRX3_l02_n01_AtbZIP18_EriAmpPoolHmB-B_aj2
        18.1%
        36%
        2.0
        HYN72DRX3_l02_n01_AtbZIP18_KyoAmpPoolHmB-B_aj1
        23.1%
        37%
        2.9
        HYN72DRX3_l02_n01_AtbZIP18_KyoAmpPoolHmB-B_aj2
        22.0%
        37%
        2.0
        HYN72DRX3_l02_n01_AtbZIP18_LerAmpPoolHmB-B_aj1
        24.5%
        37%
        3.2
        HYN72DRX3_l02_n01_AtbZIP18_LerAmpPoolHmB-B_aj2
        23.2%
        37%
        2.2
        HYN72DRX3_l02_n01_AtbZIP18_ShaAmpPoolHmB-B_aj1
        18.0%
        37%
        2.9
        HYN72DRX3_l02_n01_AtbZIP18_ShaAmpPoolHmB-B_aj2
        17.7%
        37%
        2.0
        HYN72DRX3_l02_n01_AtbZIP21_An1AmpPoolHmB-B_aj1
        0.0%
        32%
        0.0
        HYN72DRX3_l02_n01_AtbZIP21_An1AmpPoolHmB-B_aj2
        22.4%
        37%
        1.4
        HYN72DRX3_l02_n01_AtbZIP21_C24AmpPoolHmB-B_aj1
        0.0%
        34%
        0.0
        HYN72DRX3_l02_n01_AtbZIP21_C24AmpPoolHmB-B_aj2
        22.0%
        35%
        1.3
        HYN72DRX3_l02_n01_AtbZIP21_ColAmpPoolHmB-B_aj1
        0.0%
        43%
        0.0
        HYN72DRX3_l02_n01_AtbZIP21_ColAmpPoolHmB-B_aj2
        23.8%
        36%
        1.8
        HYN72DRX3_l02_n01_AtbZIP21_CviAmpPoolHmB-B_aj1
        0.0%
        28%
        0.0
        HYN72DRX3_l02_n01_AtbZIP21_CviAmpPoolHmB-B_aj2
        22.8%
        36%
        1.3
        HYN72DRX3_l02_n01_AtbZIP21_EriAmpPoolHmB-B_aj1
        0.0%
        26%
        0.0
        HYN72DRX3_l02_n01_AtbZIP21_EriAmpPoolHmB-B_aj2
        19.3%
        36%
        1.2
        HYN72DRX3_l02_n01_AtbZIP21_KyoAmpPoolHmB-B_aj1
        0.0%
        27%
        0.0
        HYN72DRX3_l02_n01_AtbZIP21_KyoAmpPoolHmB-B_aj2
        22.8%
        36%
        1.3
        HYN72DRX3_l02_n01_AtbZIP21_LerAmpPoolHmB-B_aj1
        0.0%
        34%
        0.0
        HYN72DRX3_l02_n01_AtbZIP21_LerAmpPoolHmB-B_aj2
        23.2%
        36%
        1.4
        HYN72DRX3_l02_n01_AtbZIP21_ShaAmpPoolHmB-B_aj1
        0.0%
        38%
        0.0
        HYN72DRX3_l02_n01_AtbZIP21_ShaAmpPoolHmB-B_aj2
        20.3%
        36%
        1.3
        HYN72DRX3_l02_n01_AtbZIP22_An1AmpPoolHmB-B_aj1
        20.5%
        35%
        2.6
        HYN72DRX3_l02_n01_AtbZIP22_An1AmpPoolHmB-B_aj2
        21.6%
        35%
        1.8
        HYN72DRX3_l02_n01_AtbZIP22_C24AmpPoolHmB-B_aj1
        20.0%
        34%
        3.0
        HYN72DRX3_l02_n01_AtbZIP22_C24AmpPoolHmB-B_aj2
        21.6%
        34%
        2.1
        HYN72DRX3_l02_n01_AtbZIP22_ColAmpPoolHmB-B_aj1
        22.2%
        35%
        3.6
        HYN72DRX3_l02_n01_AtbZIP22_ColAmpPoolHmB-B_aj2
        23.5%
        35%
        2.5
        HYN72DRX3_l02_n01_AtbZIP22_CviAmpPoolHmB-B_aj1
        20.8%
        35%
        2.6
        HYN72DRX3_l02_n01_AtbZIP22_CviAmpPoolHmB-B_aj2
        22.2%
        35%
        1.9
        HYN72DRX3_l02_n01_AtbZIP22_EriAmpPoolHmB-B_aj1
        16.9%
        34%
        2.5
        HYN72DRX3_l02_n01_AtbZIP22_EriAmpPoolHmB-B_aj2
        18.8%
        34%
        1.7
        HYN72DRX3_l02_n01_AtbZIP22_KyoAmpPoolHmB-B_aj1
        20.2%
        35%
        2.6
        HYN72DRX3_l02_n01_AtbZIP22_KyoAmpPoolHmB-B_aj2
        21.7%
        35%
        1.8
        HYN72DRX3_l02_n01_AtbZIP22_LerAmpPoolHmB-B_aj1
        21.8%
        35%
        2.7
        HYN72DRX3_l02_n01_AtbZIP22_LerAmpPoolHmB-B_aj2
        23.0%
        35%
        1.9
        HYN72DRX3_l02_n01_AtbZIP22_ShaAmpPoolHmB-B_aj1
        16.3%
        35%
        2.8
        HYN72DRX3_l02_n01_AtbZIP22_ShaAmpPoolHmB-B_aj2
        18.9%
        35%
        1.9
        HYN72DRX3_l02_n01_AtbZIP23_An1AmpPoolHmB-B_aj1
        28.8%
        36%
        3.6
        HYN72DRX3_l02_n01_AtbZIP23_An1AmpPoolHmB-B_aj2
        26.4%
        36%
        1.7
        HYN72DRX3_l02_n01_AtbZIP23_C24AmpPoolHmB-B_aj1
        28.1%
        35%
        3.2
        HYN72DRX3_l02_n01_AtbZIP23_C24AmpPoolHmB-B_aj2
        25.7%
        35%
        1.5
        HYN72DRX3_l02_n01_AtbZIP23_ColAmpPoolHmB-B_aj1
        30.8%
        36%
        4.8
        HYN72DRX3_l02_n01_AtbZIP23_ColAmpPoolHmB-B_aj2
        27.6%
        36%
        2.2
        HYN72DRX3_l02_n01_AtbZIP23_CviAmpPoolHmB-B_aj1
        28.9%
        36%
        3.5
        HYN72DRX3_l02_n01_AtbZIP23_CviAmpPoolHmB-B_aj2
        26.4%
        36%
        1.6
        HYN72DRX3_l02_n01_AtbZIP23_EriAmpPoolHmB-B_aj1
        24.7%
        35%
        3.0
        HYN72DRX3_l02_n01_AtbZIP23_EriAmpPoolHmB-B_aj2
        22.9%
        35%
        1.4
        HYN72DRX3_l02_n01_AtbZIP23_KyoAmpPoolHmB-B_aj1
        29.2%
        35%
        3.3
        HYN72DRX3_l02_n01_AtbZIP23_KyoAmpPoolHmB-B_aj2
        26.6%
        36%
        1.5
        HYN72DRX3_l02_n01_AtbZIP23_LerAmpPoolHmB-B_aj1
        29.9%
        36%
        3.4
        HYN72DRX3_l02_n01_AtbZIP23_LerAmpPoolHmB-B_aj2
        26.9%
        36%
        1.7
        HYN72DRX3_l02_n01_AtbZIP23_ShaAmpPoolHmB-B_aj1
        24.6%
        35%
        3.3
        HYN72DRX3_l02_n01_AtbZIP23_ShaAmpPoolHmB-B_aj2
        23.7%
        36%
        1.5
        HYN72DRX3_l02_n01_AtbZIP26_An1AmpPoolHmB-B_aj
        26.0%
        36%
        2.0
        HYN72DRX3_l02_n01_AtbZIP26_C24AmpPoolHmB-B_aj
        25.5%
        35%
        1.9
        HYN72DRX3_l02_n01_AtbZIP26_ColAmpPoolHmB-B_aj
        27.5%
        36%
        2.7
        HYN72DRX3_l02_n01_AtbZIP26_CviAmpPoolHmB-B_aj
        26.1%
        36%
        2.0
        HYN72DRX3_l02_n01_AtbZIP26_EriAmpPoolHmB-B_aj
        22.4%
        35%
        1.7
        HYN72DRX3_l02_n01_AtbZIP26_KyoAmpPoolHmB-B_aj
        26.2%
        36%
        1.9
        HYN72DRX3_l02_n01_AtbZIP26_LerAmpPoolHmB-B_aj
        27.0%
        36%
        2.0
        HYN72DRX3_l02_n01_AtbZIP26_ShaAmpPoolHmB-B_aj
        23.2%
        36%
        2.0
        HYN72DRX3_l02_n01_AtbZIP2_An1AmpPoolHmB-B_aj1
        52.3%
        36%
        4.9
        HYN72DRX3_l02_n01_AtbZIP2_An1AmpPoolHmB-B_aj2
        37.7%
        36%
        2.2
        HYN72DRX3_l02_n01_AtbZIP2_C24AmpPoolHmB-B_aj1
        53.3%
        35%
        5.6
        HYN72DRX3_l02_n01_AtbZIP2_C24AmpPoolHmB-B_aj2
        38.4%
        35%
        2.5
        HYN72DRX3_l02_n01_AtbZIP2_ColAmpPoolHmB-B_aj1
        54.3%
        36%
        6.9
        HYN72DRX3_l02_n01_AtbZIP2_ColAmpPoolHmB-B_aj2
        39.5%
        36%
        3.1
        HYN72DRX3_l02_n01_AtbZIP2_CviAmpPoolHmB-B_aj1
        52.2%
        36%
        5.1
        HYN72DRX3_l02_n01_AtbZIP2_CviAmpPoolHmB-B_aj2
        37.9%
        36%
        2.3
        HYN72DRX3_l02_n01_AtbZIP2_EriAmpPoolHmB-B_aj1
        50.4%
        35%
        4.6
        HYN72DRX3_l02_n01_AtbZIP2_EriAmpPoolHmB-B_aj2
        35.2%
        35%
        2.0
        HYN72DRX3_l02_n01_AtbZIP2_KyoAmpPoolHmB-B_aj1
        53.7%
        36%
        5.1
        HYN72DRX3_l02_n01_AtbZIP2_KyoAmpPoolHmB-B_aj2
        38.9%
        36%
        2.3
        HYN72DRX3_l02_n01_AtbZIP2_LerAmpPoolHmB-B_aj1
        53.5%
        36%
        5.2
        HYN72DRX3_l02_n01_AtbZIP2_LerAmpPoolHmB-B_aj2
        39.0%
        36%
        2.3
        HYN72DRX3_l02_n01_AtbZIP2_ShaAmpPoolHmB-B_aj1
        50.4%
        35%
        5.1
        HYN72DRX3_l02_n01_AtbZIP2_ShaAmpPoolHmB-B_aj2
        35.9%
        36%
        2.3
        HYN72DRX3_l02_n01_AtbZIP55_An1AmpPoolHmB-B_aj1
        18.2%
        38%
        2.5
        HYN72DRX3_l02_n01_AtbZIP55_An1AmpPoolHmB-B_aj2
        19.1%
        39%
        2.3
        HYN72DRX3_l02_n01_AtbZIP55_C24AmpPoolHmB-B_aj1
        19.1%
        37%
        3.0
        HYN72DRX3_l02_n01_AtbZIP55_C24AmpPoolHmB-B_aj2
        19.4%
        38%
        2.4
        HYN72DRX3_l02_n01_AtbZIP55_ColAmpPoolHmB-B_aj1
        20.4%
        37%
        3.7
        HYN72DRX3_l02_n01_AtbZIP55_ColAmpPoolHmB-B_aj2
        21.0%
        38%
        3.2
        HYN72DRX3_l02_n01_AtbZIP55_CviAmpPoolHmB-B_aj1
        19.2%
        37%
        2.9
        HYN72DRX3_l02_n01_AtbZIP55_CviAmpPoolHmB-B_aj2
        19.6%
        38%
        2.6
        HYN72DRX3_l02_n01_AtbZIP55_EriAmpPoolHmB-B_aj1
        15.5%
        37%
        2.7
        HYN72DRX3_l02_n01_AtbZIP55_EriAmpPoolHmB-B_aj2
        15.9%
        38%
        2.4
        HYN72DRX3_l02_n01_AtbZIP55_KyoAmpPoolHmB-B_aj1
        18.9%
        37%
        2.5
        HYN72DRX3_l02_n01_AtbZIP55_KyoAmpPoolHmB-B_aj2
        19.6%
        38%
        2.2
        HYN72DRX3_l02_n01_AtbZIP55_LerAmpPoolHmB-B_aj1
        19.7%
        37%
        2.6
        HYN72DRX3_l02_n01_AtbZIP55_LerAmpPoolHmB-B_aj2
        20.3%
        38%
        2.3
        HYN72DRX3_l02_n01_AtbZIP55_ShaAmpPoolHmB-B_aj1
        15.8%
        37%
        3.0
        HYN72DRX3_l02_n01_AtbZIP55_ShaAmpPoolHmB-B_aj2
        16.6%
        38%
        2.6
        HYN72DRX3_l02_n01_pIXHALO_An1AmpPoolHmB-B_aj
        23.9%
        37%
        1.3
        HYN72DRX3_l02_n01_pIXHALO_C24AmpPoolHmB-B_aj
        24.0%
        36%
        1.3
        HYN72DRX3_l02_n01_pIXHALO_ColAmpPoolHmB-B_aj
        25.4%
        36%
        1.7
        HYN72DRX3_l02_n01_pIXHALO_CviAmpPoolHmB-B_aj
        24.1%
        36%
        1.3
        HYN72DRX3_l02_n01_pIXHALO_EriAmpPoolHmB-B_aj
        20.8%
        36%
        1.2
        HYN72DRX3_l02_n01_pIXHALO_KyoAmpPoolHmB-B_aj
        24.5%
        36%
        1.3
        HYN72DRX3_l02_n01_pIXHALO_LerAmpPoolHmB-B_aj
        24.8%
        36%
        1.3
        HYN72DRX3_l02_n01_pIXHALO_ShaAmpPoolHmB-B_aj
        21.8%
        36%
        1.3
        HYN72DRX3_l02_n01_undetermined
        67.1%
        40%
        42.1
        HYN72DRX3_l02_n02_AtbZIP16_An1AmpPoolHmB-B_aj1
        19.7%
        37%
        2.6
        HYN72DRX3_l02_n02_AtbZIP16_An1AmpPoolHmB-B_aj2
        20.2%
        37%
        2.6
        HYN72DRX3_l02_n02_AtbZIP16_C24AmpPoolHmB-B_aj1
        19.5%
        36%
        2.7
        HYN72DRX3_l02_n02_AtbZIP16_C24AmpPoolHmB-B_aj2
        20.3%
        37%
        2.8
        HYN72DRX3_l02_n02_AtbZIP16_ColAmpPoolHmB-B_a2
        21.8%
        37%
        3.5
        HYN72DRX3_l02_n02_AtbZIP16_ColAmpPoolHmB-B_aj1
        21.5%
        36%
        3.5
        HYN72DRX3_l02_n02_AtbZIP16_CviAmpPoolHmB-B_aj1
        20.4%
        36%
        2.7
        HYN72DRX3_l02_n02_AtbZIP16_CviAmpPoolHmB-B_aj2
        21.1%
        37%
        2.7
        HYN72DRX3_l02_n02_AtbZIP16_EriAmpPoolHmB-B_aj1
        16.3%
        36%
        2.4
        HYN72DRX3_l02_n02_AtbZIP16_EriAmpPoolHmB-B_aj2
        16.3%
        37%
        2.4
        HYN72DRX3_l02_n02_AtbZIP16_KyoAmpPoolHmB-B_aj1
        19.9%
        36%
        2.5
        HYN72DRX3_l02_n02_AtbZIP16_KyoAmpPoolHmB-B_aj2
        20.3%
        37%
        2.6
        HYN72DRX3_l02_n02_AtbZIP16_LerAmpPoolHmB-B_aj1
        20.9%
        37%
        2.6
        HYN72DRX3_l02_n02_AtbZIP16_LerAmpPoolHmB-B_aj2
        21.5%
        37%
        2.7
        HYN72DRX3_l02_n02_AtbZIP16_ShaAmpPoolHmB-B_aj1
        16.4%
        36%
        2.7
        HYN72DRX3_l02_n02_AtbZIP16_ShaAmpPoolHmB-B_aj2
        17.1%
        37%
        2.8
        HYN72DRX3_l02_n02_AtbZIP18_An1AmpPoolHmB-B_aj1
        22.8%
        37%
        3.6
        HYN72DRX3_l02_n02_AtbZIP18_An1AmpPoolHmB-B_aj2
        21.7%
        37%
        2.5
        HYN72DRX3_l02_n02_AtbZIP18_C24AmpPoolHmB-B_aj1
        21.6%
        36%
        3.0
        HYN72DRX3_l02_n02_AtbZIP18_C24AmpPoolHmB-B_aj2
        20.3%
        36%
        2.1
        HYN72DRX3_l02_n02_AtbZIP18_ColAmpPoolHmB-B_aj1
        23.2%
        37%
        3.7
        HYN72DRX3_l02_n02_AtbZIP18_ColAmpPoolHmB-B_aj2
        22.1%
        37%
        2.5
        HYN72DRX3_l02_n02_AtbZIP18_CviAmpPoolHmB-B_aj1
        22.0%
        37%
        3.0
        HYN72DRX3_l02_n02_AtbZIP18_CviAmpPoolHmB-B_aj2
        21.0%
        37%
        2.1
        HYN72DRX3_l02_n02_AtbZIP18_EriAmpPoolHmB-B_aj1
        18.1%
        36%
        2.9
        HYN72DRX3_l02_n02_AtbZIP18_EriAmpPoolHmB-B_aj2
        17.5%
        36%
        2.0
        HYN72DRX3_l02_n02_AtbZIP18_KyoAmpPoolHmB-B_aj1
        22.3%
        37%
        2.9
        HYN72DRX3_l02_n02_AtbZIP18_KyoAmpPoolHmB-B_aj2
        21.2%
        37%
        2.0
        HYN72DRX3_l02_n02_AtbZIP18_LerAmpPoolHmB-B_aj1
        23.9%
        37%
        3.2
        HYN72DRX3_l02_n02_AtbZIP18_LerAmpPoolHmB-B_aj2
        22.6%
        37%
        2.2
        HYN72DRX3_l02_n02_AtbZIP18_ShaAmpPoolHmB-B_aj1
        17.3%
        37%
        2.9
        HYN72DRX3_l02_n02_AtbZIP18_ShaAmpPoolHmB-B_aj2
        17.1%
        37%
        2.0
        HYN72DRX3_l02_n02_AtbZIP21_An1AmpPoolHmB-B_aj1
        0.0%
        21%
        0.0
        HYN72DRX3_l02_n02_AtbZIP21_An1AmpPoolHmB-B_aj2
        21.6%
        36%
        1.4
        HYN72DRX3_l02_n02_AtbZIP21_C24AmpPoolHmB-B_aj1
        0.0%
        33%
        0.0
        HYN72DRX3_l02_n02_AtbZIP21_C24AmpPoolHmB-B_aj2
        21.5%
        35%
        1.3
        HYN72DRX3_l02_n02_AtbZIP21_ColAmpPoolHmB-B_aj1
        0.0%
        28%
        0.0
        HYN72DRX3_l02_n02_AtbZIP21_ColAmpPoolHmB-B_aj2
        23.0%
        36%
        1.8
        HYN72DRX3_l02_n02_AtbZIP21_CviAmpPoolHmB-B_aj1
        0.0%
        30%
        0.0
        HYN72DRX3_l02_n02_AtbZIP21_CviAmpPoolHmB-B_aj2
        21.9%
        36%
        1.3
        HYN72DRX3_l02_n02_AtbZIP21_EriAmpPoolHmB-B_aj1
        0.0%
        30%
        0.0
        HYN72DRX3_l02_n02_AtbZIP21_EriAmpPoolHmB-B_aj2
        18.7%
        36%
        1.2
        HYN72DRX3_l02_n02_AtbZIP21_KyoAmpPoolHmB-B_aj1
        0.0%
        27%
        0.0
        HYN72DRX3_l02_n02_AtbZIP21_KyoAmpPoolHmB-B_aj2
        21.9%
        36%
        1.3
        HYN72DRX3_l02_n02_AtbZIP21_LerAmpPoolHmB-B_aj1
        0.0%
        40%
        0.0
        HYN72DRX3_l02_n02_AtbZIP21_LerAmpPoolHmB-B_aj2
        22.5%
        36%
        1.4
        HYN72DRX3_l02_n02_AtbZIP21_ShaAmpPoolHmB-B_aj1
        0.0%
        39%
        0.0
        HYN72DRX3_l02_n02_AtbZIP21_ShaAmpPoolHmB-B_aj2
        19.4%
        36%
        1.3
        HYN72DRX3_l02_n02_AtbZIP22_An1AmpPoolHmB-B_aj1
        19.8%
        35%
        2.6
        HYN72DRX3_l02_n02_AtbZIP22_An1AmpPoolHmB-B_aj2
        21.3%
        35%
        1.8
        HYN72DRX3_l02_n02_AtbZIP22_C24AmpPoolHmB-B_aj1
        19.7%
        34%
        3.0
        HYN72DRX3_l02_n02_AtbZIP22_C24AmpPoolHmB-B_aj2
        21.5%
        34%
        2.1
        HYN72DRX3_l02_n02_AtbZIP22_ColAmpPoolHmB-B_aj1
        21.8%
        35%
        3.6
        HYN72DRX3_l02_n02_AtbZIP22_ColAmpPoolHmB-B_aj2
        23.4%
        35%
        2.5
        HYN72DRX3_l02_n02_AtbZIP22_CviAmpPoolHmB-B_aj1
        20.3%
        35%
        2.6
        HYN72DRX3_l02_n02_AtbZIP22_CviAmpPoolHmB-B_aj2
        21.9%
        35%
        1.9
        HYN72DRX3_l02_n02_AtbZIP22_EriAmpPoolHmB-B_aj1
        16.5%
        34%
        2.5
        HYN72DRX3_l02_n02_AtbZIP22_EriAmpPoolHmB-B_aj2
        18.6%
        34%
        1.7
        HYN72DRX3_l02_n02_AtbZIP22_KyoAmpPoolHmB-B_aj1
        19.7%
        35%
        2.6
        HYN72DRX3_l02_n02_AtbZIP22_KyoAmpPoolHmB-B_aj2
        21.4%
        35%
        1.8
        HYN72DRX3_l02_n02_AtbZIP22_LerAmpPoolHmB-B_aj1
        21.2%
        35%
        2.7
        HYN72DRX3_l02_n02_AtbZIP22_LerAmpPoolHmB-B_aj2
        22.7%
        35%
        1.9
        HYN72DRX3_l02_n02_AtbZIP22_ShaAmpPoolHmB-B_aj1
        15.9%
        35%
        2.8
        HYN72DRX3_l02_n02_AtbZIP22_ShaAmpPoolHmB-B_aj2
        18.5%
        35%
        1.9
        HYN72DRX3_l02_n02_AtbZIP23_An1AmpPoolHmB-B_aj1
        27.9%
        36%
        3.6
        HYN72DRX3_l02_n02_AtbZIP23_An1AmpPoolHmB-B_aj2
        25.4%
        36%
        1.7
        HYN72DRX3_l02_n02_AtbZIP23_C24AmpPoolHmB-B_aj1
        27.6%
        35%
        3.2
        HYN72DRX3_l02_n02_AtbZIP23_C24AmpPoolHmB-B_aj2
        25.2%
        35%
        1.5
        HYN72DRX3_l02_n02_AtbZIP23_ColAmpPoolHmB-B_aj1
        29.9%
        36%
        4.8
        HYN72DRX3_l02_n02_AtbZIP23_ColAmpPoolHmB-B_aj2
        27.0%
        36%
        2.2
        HYN72DRX3_l02_n02_AtbZIP23_CviAmpPoolHmB-B_aj1
        28.2%
        36%
        3.5
        HYN72DRX3_l02_n02_AtbZIP23_CviAmpPoolHmB-B_aj2
        25.6%
        36%
        1.6
        HYN72DRX3_l02_n02_AtbZIP23_EriAmpPoolHmB-B_aj1
        24.1%
        35%
        3.0
        HYN72DRX3_l02_n02_AtbZIP23_EriAmpPoolHmB-B_aj2
        22.2%
        35%
        1.4
        HYN72DRX3_l02_n02_AtbZIP23_KyoAmpPoolHmB-B_aj1
        28.3%
        36%
        3.3
        HYN72DRX3_l02_n02_AtbZIP23_KyoAmpPoolHmB-B_aj2
        25.6%
        36%
        1.5
        HYN72DRX3_l02_n02_AtbZIP23_LerAmpPoolHmB-B_aj1
        29.2%
        36%
        3.4
        HYN72DRX3_l02_n02_AtbZIP23_LerAmpPoolHmB-B_aj2
        26.1%
        36%
        1.7
        HYN72DRX3_l02_n02_AtbZIP23_ShaAmpPoolHmB-B_aj1
        23.9%
        35%
        3.3
        HYN72DRX3_l02_n02_AtbZIP23_ShaAmpPoolHmB-B_aj2
        22.8%
        36%
        1.5
        HYN72DRX3_l02_n02_AtbZIP26_An1AmpPoolHmB-B_aj
        25.2%
        36%
        2.0
        HYN72DRX3_l02_n02_AtbZIP26_C24AmpPoolHmB-B_aj
        25.3%
        35%
        1.9
        HYN72DRX3_l02_n02_AtbZIP26_ColAmpPoolHmB-B_aj
        26.9%
        36%
        2.7
        HYN72DRX3_l02_n02_AtbZIP26_CviAmpPoolHmB-B_aj
        25.6%
        36%
        2.0
        HYN72DRX3_l02_n02_AtbZIP26_EriAmpPoolHmB-B_aj
        22.0%
        35%
        1.7
        HYN72DRX3_l02_n02_AtbZIP26_KyoAmpPoolHmB-B_aj
        25.7%
        36%
        1.9
        HYN72DRX3_l02_n02_AtbZIP26_LerAmpPoolHmB-B_aj
        26.5%
        36%
        2.0
        HYN72DRX3_l02_n02_AtbZIP26_ShaAmpPoolHmB-B_aj
        22.5%
        36%
        2.0
        HYN72DRX3_l02_n02_AtbZIP2_An1AmpPoolHmB-B_aj1
        50.8%
        36%
        4.9
        HYN72DRX3_l02_n02_AtbZIP2_An1AmpPoolHmB-B_aj2
        36.8%
        36%
        2.2
        HYN72DRX3_l02_n02_AtbZIP2_C24AmpPoolHmB-B_aj1
        52.8%
        35%
        5.6
        HYN72DRX3_l02_n02_AtbZIP2_C24AmpPoolHmB-B_aj2
        37.9%
        35%
        2.5
        HYN72DRX3_l02_n02_AtbZIP2_ColAmpPoolHmB-B_aj1
        53.2%
        36%
        6.9
        HYN72DRX3_l02_n02_AtbZIP2_ColAmpPoolHmB-B_aj2
        38.9%
        36%
        3.1
        HYN72DRX3_l02_n02_AtbZIP2_CviAmpPoolHmB-B_aj1
        51.2%
        36%
        5.1
        HYN72DRX3_l02_n02_AtbZIP2_CviAmpPoolHmB-B_aj2
        37.2%
        36%
        2.3
        HYN72DRX3_l02_n02_AtbZIP2_EriAmpPoolHmB-B_aj1
        49.5%
        35%
        4.6
        HYN72DRX3_l02_n02_AtbZIP2_EriAmpPoolHmB-B_aj2
        34.5%
        35%
        2.0
        HYN72DRX3_l02_n02_AtbZIP2_KyoAmpPoolHmB-B_aj1
        52.3%
        36%
        5.1
        HYN72DRX3_l02_n02_AtbZIP2_KyoAmpPoolHmB-B_aj2
        38.0%
        36%
        2.3
        HYN72DRX3_l02_n02_AtbZIP2_LerAmpPoolHmB-B_aj1
        52.3%
        36%
        5.2
        HYN72DRX3_l02_n02_AtbZIP2_LerAmpPoolHmB-B_aj2
        38.2%
        36%
        2.3
        HYN72DRX3_l02_n02_AtbZIP2_ShaAmpPoolHmB-B_aj1
        49.3%
        35%
        5.1
        HYN72DRX3_l02_n02_AtbZIP2_ShaAmpPoolHmB-B_aj2
        35.1%
        36%
        2.3
        HYN72DRX3_l02_n02_AtbZIP55_An1AmpPoolHmB-B_aj1
        17.3%
        38%
        2.5
        HYN72DRX3_l02_n02_AtbZIP55_An1AmpPoolHmB-B_aj2
        18.3%
        38%
        2.3
        HYN72DRX3_l02_n02_AtbZIP55_C24AmpPoolHmB-B_aj1
        18.8%
        37%
        3.0
        HYN72DRX3_l02_n02_AtbZIP55_C24AmpPoolHmB-B_aj2
        19.1%
        38%
        2.4
        HYN72DRX3_l02_n02_AtbZIP55_ColAmpPoolHmB-B_aj1
        19.9%
        37%
        3.7
        HYN72DRX3_l02_n02_AtbZIP55_ColAmpPoolHmB-B_aj2
        20.1%
        38%
        3.2
        HYN72DRX3_l02_n02_AtbZIP55_CviAmpPoolHmB-B_aj1
        18.6%
        37%
        2.9
        HYN72DRX3_l02_n02_AtbZIP55_CviAmpPoolHmB-B_aj2
        19.0%
        38%
        2.6
        HYN72DRX3_l02_n02_AtbZIP55_EriAmpPoolHmB-B_aj1
        14.9%
        37%
        2.7
        HYN72DRX3_l02_n02_AtbZIP55_EriAmpPoolHmB-B_aj2
        15.4%
        37%
        2.4
        HYN72DRX3_l02_n02_AtbZIP55_KyoAmpPoolHmB-B_aj1
        18.3%
        37%
        2.5
        HYN72DRX3_l02_n02_AtbZIP55_KyoAmpPoolHmB-B_aj2
        18.8%
        38%
        2.2
        HYN72DRX3_l02_n02_AtbZIP55_LerAmpPoolHmB-B_aj1
        19.0%
        37%
        2.6
        HYN72DRX3_l02_n02_AtbZIP55_LerAmpPoolHmB-B_aj2
        19.5%
        38%
        2.3
        HYN72DRX3_l02_n02_AtbZIP55_ShaAmpPoolHmB-B_aj1
        15.1%
        37%
        3.0
        HYN72DRX3_l02_n02_AtbZIP55_ShaAmpPoolHmB-B_aj2
        15.9%
        38%
        2.6
        HYN72DRX3_l02_n02_pIXHALO_An1AmpPoolHmB-B_aj
        23.1%
        37%
        1.3
        HYN72DRX3_l02_n02_pIXHALO_C24AmpPoolHmB-B_aj
        23.7%
        36%
        1.3
        HYN72DRX3_l02_n02_pIXHALO_ColAmpPoolHmB-B_aj
        24.9%
        36%
        1.7
        HYN72DRX3_l02_n02_pIXHALO_CviAmpPoolHmB-B_aj
        23.5%
        36%
        1.3
        HYN72DRX3_l02_n02_pIXHALO_EriAmpPoolHmB-B_aj
        20.5%
        36%
        1.2
        HYN72DRX3_l02_n02_pIXHALO_KyoAmpPoolHmB-B_aj
        23.9%
        36%
        1.3
        HYN72DRX3_l02_n02_pIXHALO_LerAmpPoolHmB-B_aj
        24.2%
        36%
        1.3
        HYN72DRX3_l02_n02_pIXHALO_ShaAmpPoolHmB-B_aj
        21.3%
        36%
        1.3
        HYN72DRX3_l02_n02_undetermined
        63.4%
        41%
        42.1

        Demultiplexing Report


        Total Read Count: Total number of PF (Passing Filter) reads in this library.
        Portion: The proportion of reads that represent the individual library in the entire Library Pool.

        Showing 129/129 rows and 2/2 columns.
        LibraryTotal Read CountPortion (%)
        undetermined_library
        42090463
        12.0
        AtbZIP18_ColAmpPoolHmB-B_aj1
        3702749
        1.1
        AtbZIP18_C24AmpPoolHmB-B_aj1
        3032938
        0.9
        AtbZIP18_EriAmpPoolHmB-B_aj1
        2874936
        0.8
        AtbZIP18_CviAmpPoolHmB-B_aj1
        3018227
        0.9
        AtbZIP18_ShaAmpPoolHmB-B_aj1
        2892768
        0.8
        AtbZIP18_KyoAmpPoolHmB-B_aj1
        2918001
        0.8
        AtbZIP18_LerAmpPoolHmB-B_aj1
        3150105
        0.9
        AtbZIP18_An1AmpPoolHmB-B_aj1
        3558232
        1.0
        AtbZIP18_ColAmpPoolHmB-B_aj2
        2548025
        0.7
        AtbZIP18_C24AmpPoolHmB-B_aj2
        2072655
        0.6
        AtbZIP18_EriAmpPoolHmB-B_aj2
        1997811
        0.6
        AtbZIP18_CviAmpPoolHmB-B_aj2
        2102769
        0.6
        AtbZIP18_ShaAmpPoolHmB-B_aj2
        2015616
        0.6
        AtbZIP18_KyoAmpPoolHmB-B_aj2
        2012894
        0.6
        AtbZIP18_LerAmpPoolHmB-B_aj2
        2188416
        0.6
        AtbZIP18_An1AmpPoolHmB-B_aj2
        2465564
        0.7
        AtbZIP16_ColAmpPoolHmB-B_aj1
        3513538
        1.0
        AtbZIP16_C24AmpPoolHmB-B_aj1
        2688840
        0.8
        AtbZIP16_EriAmpPoolHmB-B_aj1
        2440186
        0.7
        AtbZIP16_CviAmpPoolHmB-B_aj1
        2681112
        0.8
        AtbZIP16_ShaAmpPoolHmB-B_aj1
        2735362
        0.8
        AtbZIP16_KyoAmpPoolHmB-B_aj1
        2523574
        0.7
        AtbZIP16_LerAmpPoolHmB-B_aj1
        2575799
        0.7
        AtbZIP16_An1AmpPoolHmB-B_aj1
        2588577
        0.7
        AtbZIP16_ColAmpPoolHmB-B_a2
        3482998
        1.0
        AtbZIP16_C24AmpPoolHmB-B_aj2
        2820874
        0.8
        AtbZIP16_EriAmpPoolHmB-B_aj2
        2408547
        0.7
        AtbZIP16_CviAmpPoolHmB-B_aj2
        2704505
        0.8
        AtbZIP16_ShaAmpPoolHmB-B_aj2
        2797370
        0.8
        AtbZIP16_KyoAmpPoolHmB-B_aj2
        2551528
        0.7
        AtbZIP16_LerAmpPoolHmB-B_aj2
        2685245
        0.8
        AtbZIP16_An1AmpPoolHmB-B_aj2
        2582829
        0.7
        AtbZIP2_ColAmpPoolHmB-B_aj1
        6909600
        2.0
        AtbZIP2_C24AmpPoolHmB-B_aj1
        5558982
        1.6
        AtbZIP2_EriAmpPoolHmB-B_aj1
        4583240
        1.3
        AtbZIP2_CviAmpPoolHmB-B_aj1
        5091471
        1.5
        AtbZIP2_ShaAmpPoolHmB-B_aj1
        5129759
        1.5
        AtbZIP2_KyoAmpPoolHmB-B_aj1
        5104774
        1.5
        AtbZIP2_LerAmpPoolHmB-B_aj1
        5212567
        1.5
        AtbZIP2_An1AmpPoolHmB-B_aj1
        4908720
        1.4
        AtbZIP2_ColAmpPoolHmB-B_aj2
        3058581
        0.9
        AtbZIP2_C24AmpPoolHmB-B_aj2
        2479526
        0.7
        AtbZIP2_EriAmpPoolHmB-B_aj2
        2026403
        0.6
        AtbZIP2_CviAmpPoolHmB-B_aj2
        2263032
        0.6
        AtbZIP2_ShaAmpPoolHmB-B_aj2
        2295886
        0.7
        AtbZIP2_KyoAmpPoolHmB-B_aj2
        2256049
        0.6
        AtbZIP2_LerAmpPoolHmB-B_aj2
        2333020
        0.7
        AtbZIP2_An1AmpPoolHmB-B_aj2
        2180316
        0.6
        AtbZIP23_ColAmpPoolHmB-B_aj1
        4840051
        1.4
        AtbZIP23_C24AmpPoolHmB-B_aj1
        3233383
        0.9
        AtbZIP23_EriAmpPoolHmB-B_aj1
        2999653
        0.9
        AtbZIP23_CviAmpPoolHmB-B_aj1
        3471143
        1.0
        AtbZIP23_ShaAmpPoolHmB-B_aj1
        3290713
        0.9
        AtbZIP23_KyoAmpPoolHmB-B_aj1
        3325786
        1.0
        AtbZIP23_LerAmpPoolHmB-B_aj1
        3449210
        1.0
        AtbZIP23_An1AmpPoolHmB-B_aj1
        3556495
        1.0
        AtbZIP23_ColAmpPoolHmB-B_aj2
        2243841
        0.6
        AtbZIP23_C24AmpPoolHmB-B_aj2
        1462227
        0.4
        AtbZIP23_EriAmpPoolHmB-B_aj2
        1370344
        0.4
        AtbZIP23_CviAmpPoolHmB-B_aj2
        1637126
        0.5
        AtbZIP23_ShaAmpPoolHmB-B_aj2
        1522941
        0.4
        AtbZIP23_KyoAmpPoolHmB-B_aj2
        1537796
        0.4
        AtbZIP23_LerAmpPoolHmB-B_aj2
        1671698
        0.5
        AtbZIP23_An1AmpPoolHmB-B_aj2
        1675140
        0.5
        AtbZIP21_ColAmpPoolHmB-B_aj1
        3.0
        0.0
        AtbZIP21_C24AmpPoolHmB-B_aj1
        5.0
        0.0
        AtbZIP21_EriAmpPoolHmB-B_aj1
        2.0
        0.0
        AtbZIP21_CviAmpPoolHmB-B_aj1
        3.0
        0.0
        AtbZIP21_ShaAmpPoolHmB-B_aj1
        6.0
        0.0
        AtbZIP21_KyoAmpPoolHmB-B_aj1
        3.0
        0.0
        AtbZIP21_LerAmpPoolHmB-B_aj1
        2.0
        0.0
        AtbZIP21_An1AmpPoolHmB-B_aj1
        3.0
        0.0
        AtbZIP21_ColAmpPoolHmB-B_aj2
        1814057
        0.5
        AtbZIP21_C24AmpPoolHmB-B_aj2
        1282760
        0.4
        AtbZIP21_EriAmpPoolHmB-B_aj2
        1172751
        0.3
        AtbZIP21_CviAmpPoolHmB-B_aj2
        1346566
        0.4
        AtbZIP21_ShaAmpPoolHmB-B_aj2
        1306663
        0.4
        AtbZIP21_KyoAmpPoolHmB-B_aj2
        1309289
        0.4
        AtbZIP21_LerAmpPoolHmB-B_aj2
        1377472
        0.4
        AtbZIP21_An1AmpPoolHmB-B_aj2
        1365132
        0.4
        AtbZIP22_ColAmpPoolHmB-B_aj1
        3554996
        1.0
        AtbZIP22_C24AmpPoolHmB-B_aj1
        2972726
        0.8
        AtbZIP22_EriAmpPoolHmB-B_aj1
        2466685
        0.7
        AtbZIP22_CviAmpPoolHmB-B_aj1
        2637970
        0.8
        AtbZIP22_ShaAmpPoolHmB-B_aj1
        2797547
        0.8
        AtbZIP22_KyoAmpPoolHmB-B_aj1
        2585602
        0.7
        AtbZIP22_LerAmpPoolHmB-B_aj1
        2666731
        0.8
        AtbZIP22_An1AmpPoolHmB-B_aj1
        2577561
        0.7
        AtbZIP22_ColAmpPoolHmB-B_aj2
        2505701
        0.7
        AtbZIP22_C24AmpPoolHmB-B_aj2
        2073289
        0.6
        AtbZIP22_EriAmpPoolHmB-B_aj2
        1719477
        0.5
        AtbZIP22_CviAmpPoolHmB-B_aj2
        1875388
        0.5
        AtbZIP22_ShaAmpPoolHmB-B_aj2
        1932599
        0.6
        AtbZIP22_KyoAmpPoolHmB-B_aj2
        1750313
        0.5
        AtbZIP22_LerAmpPoolHmB-B_aj2
        1857400
        0.5
        AtbZIP22_An1AmpPoolHmB-B_aj2
        1776001
        0.5
        AtbZIP55_ColAmpPoolHmB-B_aj1
        3702498
        1.1
        AtbZIP55_C24AmpPoolHmB-B_aj1
        3026293
        0.9
        AtbZIP55_EriAmpPoolHmB-B_aj1
        2683085
        0.8
        AtbZIP55_CviAmpPoolHmB-B_aj1
        2936016
        0.8
        AtbZIP55_ShaAmpPoolHmB-B_aj1
        2998020
        0.9
        AtbZIP55_KyoAmpPoolHmB-B_aj1
        2519632
        0.7
        AtbZIP55_LerAmpPoolHmB-B_aj1
        2620129
        0.7
        AtbZIP55_An1AmpPoolHmB-B_aj1
        2539515
        0.7
        AtbZIP55_ColAmpPoolHmB-B_aj2
        3200227
        0.9
        AtbZIP55_C24AmpPoolHmB-B_aj2
        2449586
        0.7
        AtbZIP55_EriAmpPoolHmB-B_aj2
        2365272
        0.7
        AtbZIP55_CviAmpPoolHmB-B_aj2
        2612013
        0.7
        AtbZIP55_ShaAmpPoolHmB-B_aj2
        2619597
        0.7
        AtbZIP55_KyoAmpPoolHmB-B_aj2
        2150414
        0.6
        AtbZIP55_LerAmpPoolHmB-B_aj2
        2260519
        0.6
        AtbZIP55_An1AmpPoolHmB-B_aj2
        2265526
        0.6
        AtbZIP26_ColAmpPoolHmB-B_aj
        2725440
        0.8
        AtbZIP26_C24AmpPoolHmB-B_aj
        1934469
        0.6
        AtbZIP26_EriAmpPoolHmB-B_aj
        1702512
        0.5
        AtbZIP26_CviAmpPoolHmB-B_aj
        1968590
        0.6
        AtbZIP26_ShaAmpPoolHmB-B_aj
        1950258
        0.6
        AtbZIP26_KyoAmpPoolHmB-B_aj
        1873107
        0.5
        AtbZIP26_LerAmpPoolHmB-B_aj
        2032849
        0.6
        AtbZIP26_An1AmpPoolHmB-B_aj
        1996110
        0.6
        pIXHALO_ColAmpPoolHmB-B_aj
        1743338
        0.5
        pIXHALO_C24AmpPoolHmB-B_aj
        1319199
        0.4
        pIXHALO_EriAmpPoolHmB-B_aj
        1166045
        0.3
        pIXHALO_CviAmpPoolHmB-B_aj
        1307788
        0.4
        pIXHALO_ShaAmpPoolHmB-B_aj
        1292841
        0.4
        pIXHALO_KyoAmpPoolHmB-B_aj
        1303646
        0.4
        pIXHALO_LerAmpPoolHmB-B_aj
        1342814
        0.4
        pIXHALO_An1AmpPoolHmB-B_aj
        1305382
        0.4

        Barcodes of Undetermined Reads


        We have determined the barcodes of your undetermined reads. Here are the top 20 barcodes. The full list is available here. If your libraries are dual indexed, the two indices are concatenated.

        Showing 20/20 rows and 2/2 columns.
        Barcode Sequence(s)CountFrequency (%)
        GGGGGGGGAGATCTCG
        25619513.0
        60.9
        GGGGGGGGAGGATAGG
        309720.0
        0.7
        GGGGGGGGAGTTCTCG
        284876.0
        0.7
        GGGGGGGGAGGCTATA
        196557.0
        0.5
        GGGGGGGGCTTCGCCT
        183215.0
        0.4
        GGGGGGGGACGTCCTG
        175933.0
        0.4
        GGGGGGGGTGGAAGCA
        163574.0
        0.4
        GGGGGGGGGTCAGTAC
        160216.0
        0.4
        GGGGGGGGGCCTCTAT
        159999.0
        0.4
        CGGCTAATGGGGGGGG
        135040.0
        0.3
        GGGGGGGGAGTCGAAG
        134216.0
        0.3
        GGGGGGGGCTCGTTCT
        128783.0
        0.3
        GGGGGGGGAAGGCGTA
        123289.0
        0.3
        GGGGGGGGTCTTACGG
        116223.0
        0.3
        GGGGGGGGAACCTCTC
        109160.0
        0.3
        TACGCTACGGGGGGGG
        105122.0
        0.2
        GCTATCCTGGGGGGGG
        104319.0
        0.2
        ATCGATCGGGGGGGGG
        102388.0
        0.2
        ATCCAGAGGGGGGGGG
        100427.0
        0.2
        AGAGTAGCGGGGGGGG
        100390.0
        0.2

        Run Statistics

        Showing 1/1 rows and 4/4 columns.
        LaneTotal # of Single-End ReadsTotal # PF Reads% Undetermined% PhiX Aligned
        2.0
        638337024
        349739959
        12.0
        7.4

        FastQC

        FastQC is a quality control tool for high throughput sequence data, written by Simon Andrews at the Babraham Institute in Cambridge.

        Sequence Counts

        Sequence counts for each sample. Duplicate read counts are an estimate only.

        This plot show the total number of reads, broken down into unique and duplicate if possible (only more recent versions of FastQC give duplicate info).

        You can read more about duplicate calculation in the FastQC documentation. A small part has been copied here for convenience:

        Only sequences which first appear in the first 100,000 sequences in each file are analysed. This should be enough to get a good impression for the duplication levels in the whole file. Each sequence is tracked to the end of the file to give a representative count of the overall duplication level.

        The duplication detection requires an exact sequence match over the whole length of the sequence. Any reads over 75bp in length are truncated to 50bp for this analysis.

        Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).


        Sequence Quality Histograms

        The mean quality value across each base position in the read.

        To enable multiple samples to be plotted on the same graph, only the mean quality scores are plotted (unlike the box plots seen in FastQC reports).

        Taken from the FastQC help:

        The y-axis on the graph shows the quality scores. The higher the score, the better the base call. The background of the graph divides the y axis into very good quality calls (green), calls of reasonable quality (orange), and calls of poor quality (red). The quality of calls on most platforms will degrade as the run progresses, so it is common to see base calls falling into the orange area towards the end of a read.

        Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).


        Per Sequence Quality Scores

        The number of reads with average quality scores. Shows if a subset of reads has poor quality.

        From the FastQC help:

        The per sequence quality score report allows you to see if a subset of your sequences have universally low quality values. It is often the case that a subset of sequences will have universally poor quality, however these should represent only a small percentage of the total sequences.

        Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).


        Per Base Sequence Content

        The proportion of each base position for which each of the four normal DNA bases has been called.

        To enable multiple samples to be shown in a single plot, the base composition data is shown as a heatmap. The colours represent the balance between the four bases: an even distribution should give an even muddy brown colour. Hover over the plot to see the percentage of the four bases under the cursor.

        To see the data as a line plot, as in the original FastQC graph, click on a sample track.

        From the FastQC help:

        Per Base Sequence Content plots out the proportion of each base position in a file for which each of the four normal DNA bases has been called.

        In a random library you would expect that there would be little to no difference between the different bases of a sequence run, so the lines in this plot should run parallel with each other. The relative amount of each base should reflect the overall amount of these bases in your genome, but in any case they should not be hugely imbalanced from each other.

        It's worth noting that some types of library will always produce biased sequence composition, normally at the start of the read. Libraries produced by priming using random hexamers (including nearly all RNA-Seq libraries) and those which were fragmented using transposases inherit an intrinsic bias in the positions at which reads start. This bias does not concern an absolute sequence, but instead provides enrichement of a number of different K-mers at the 5' end of the reads. Whilst this is a true technical bias, it isn't something which can be corrected by trimming and in most cases doesn't seem to adversely affect the downstream analysis.

        Click a sample row to see a line plot for that dataset.
        Rollover for sample name
        Position: -
        %T: -
        %C: -
        %A: -
        %G: -

        Per Sequence GC Content

        The average GC content of reads. Normal random library typically have a roughly normal distribution of GC content.

        From the FastQC help:

        This module measures the GC content across the whole length of each sequence in a file and compares it to a modelled normal distribution of GC content.

        In a normal random library you would expect to see a roughly normal distribution of GC content where the central peak corresponds to the overall GC content of the underlying genome. Since we don't know the the GC content of the genome the modal GC content is calculated from the observed data and used to build a reference distribution.

        An unusually shaped distribution could indicate a contaminated library or some other kinds of biased subset. A normal distribution which is shifted indicates some systematic bias which is independent of base position. If there is a systematic bias which creates a shifted normal distribution then this won't be flagged as an error by the module since it doesn't know what your genome's GC content should be.

        Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).


        Per Base N Content

        The percentage of base calls at each position for which an N was called.

        From the FastQC help:

        If a sequencer is unable to make a base call with sufficient confidence then it will normally substitute an N rather than a conventional base call. This graph shows the percentage of base calls at each position for which an N was called.

        It's not unusual to see a very low proportion of Ns appearing in a sequence, especially nearer the end of a sequence. However, if this proportion rises above a few percent it suggests that the analysis pipeline was unable to interpret the data well enough to make valid base calls.

        Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).


        Sequence Length Distribution

        All samples have sequences of a single length (101bp).

        Sequence Duplication Levels

        The relative level of duplication found for every sequence.

        From the FastQC Help:

        In a diverse library most sequences will occur only once in the final set. A low level of duplication may indicate a very high level of coverage of the target sequence, but a high level of duplication is more likely to indicate some kind of enrichment bias (eg PCR over amplification). This graph shows the degree of duplication for every sequence in a library: the relative number of sequences with different degrees of duplication.

        Only sequences which first appear in the first 100,000 sequences in each file are analysed. This should be enough to get a good impression for the duplication levels in the whole file. Each sequence is tracked to the end of the file to give a representative count of the overall duplication level.

        The duplication detection requires an exact sequence match over the whole length of the sequence. Any reads over 75bp in length are truncated to 50bp for this analysis.

        In a properly diverse library most sequences should fall into the far left of the plot in both the red and blue lines. A general level of enrichment, indicating broad oversequencing in the library will tend to flatten the lines, lowering the low end and generally raising other categories. More specific enrichments of subsets, or the presence of low complexity contaminants will tend to produce spikes towards the right of the plot.

        Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).


        Overrepresented sequences

        The total amount of overrepresented sequences found in each library.

        FastQC calculates and lists overrepresented sequences in FastQ files. It would not be possible to show this for all samples in a MultiQC report, so instead this plot shows the number of sequences categorized as over represented.

        Sometimes, a single sequence may account for a large number of reads in a dataset. To show this, the bars are split into two: the first shows the overrepresented reads that come from the single most common sequence. The second shows the total count from all remaining overrepresented sequences.

        From the FastQC Help:

        A normal high-throughput library will contain a diverse set of sequences, with no individual sequence making up a tiny fraction of the whole. Finding that a single sequence is very overrepresented in the set either means that it is highly biologically significant, or indicates that the library is contaminated, or not as diverse as you expected.

        FastQC lists all of the sequences which make up more than 0.1% of the total. To conserve memory only sequences which appear in the first 100,000 sequences are tracked to the end of the file. It is therefore possible that a sequence which is overrepresented but doesn't appear at the start of the file for some reason could be missed by this module.

        Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).


        Adapter Content

        The cumulative percentage count of the proportion of your library which has seen each of the adapter sequences at each position.

        Note that only samples with ≥ 0.1% adapter contamination are shown.

        There may be several lines per sample, as one is shown for each adapter detected in the file.

        From the FastQC Help:

        The plot shows a cumulative percentage count of the proportion of your library which has seen each of the adapter sequences at each position. Once a sequence has been seen in a read it is counted as being present right through to the end of the read so the percentages you see will only increase as the read length goes on.

        Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).


        Status Checks

        Status for each FastQC section showing whether results seem entirely normal (green), slightly abnormal (orange) or very unusual (red).

        FastQC assigns a status for each section of the report. These give a quick evaluation of whether the results of the analysis seem entirely normal (green), slightly abnormal (orange) or very unusual (red).

        It is important to stress that although the analysis results appear to give a pass/fail result, these evaluations must be taken in the context of what you expect from your library. A 'normal' sample as far as FastQC is concerned is random and diverse. Some experiments may be expected to produce libraries which are biased in particular ways. You should treat the summary evaluations therefore as pointers to where you should concentrate your attention and understand why your library may not look random and diverse.

        Specific guidance on how to interpret the output of each module can be found in the relevant report section, or in the FastQC help.

        In this heatmap, we summarise all of these into a single heatmap for a quick overview. Note that not all FastQC sections have plots in MultiQC reports, but all status checks are shown in this heatmap.

        loading..