FastQCFastQC Report
Tue 18 Jun 2019
HYN2CBCX2_l02.1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYN2CBCX2_l02.1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences151168675
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGA10235100.6770648747169347Illumina Paired End PCR Primer 2 (100% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGAG1844650.033.49575815
GCGGTTC1862550.030.61396631
CGAGATC1973200.030.46454618
AGCGGTT1875900.030.43746230
GAGCGGT1899050.029.88880229
TCGGAAG1990350.029.609823
ATCGGAA2025000.029.34421522
CCGAGAT2079950.029.14943717
AGAGCGG1975750.028.75578528
AAGAGCG2003550.028.41366427
GATCGGA2105650.028.36474621
GCCGAGA2203900.028.31305316
CGGAAGA2159750.027.2487524
AGATCGG2251700.026.58131620
GAGATCG2332950.025.8383519
GAAGAGC2432450.024.14728226
GGAAGAG2895250.020.56736825
ATGCCGA3014500.020.48270214
AATGCCG3048700.020.26250313
ACCGATC88400.019.01837322
Produced by FastQC (version 0.11.5)