FastQCFastQC Report
Tue 18 Jun 2019
HYN2CBCX2_l01.2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYN2CBCX2_l01.2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences100163629
Sequences flagged as poor quality0
Sequence length6
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNN1512892215.104207136903955No Hit
AAAAAA16836491.6808985624911812No Hit
NANNNN6934570.6923241569052974No Hit
AAAGAA5645350.5636127660670123No Hit
AATGCC4275840.4268854915390496No Hit
NAAAAA4035030.40284383066831575No Hit
AAAACA3322010.3316583108225841No Hit
AAAAAC3237390.32321013448903696No Hit
AAGAAA3155470.31503151707891897No Hit
GAAAAA3147350.3142208435758652No Hit
AAAGCC3063170.3058165953631732No Hit
AGAAAA3009060.3004144348643758No Hit
NGNNNN2970190.2965337847333786No Hit
CCCCCC2896860.2892127640463186No Hit
AAAAAG2787030.27824770606104937No Hit
AATAAA2735030.2730562008690799No Hit
AAACAA2723680.27192305502429426No Hit
AAAGCA2618600.2614322210709838No Hit
ACAAAA2618430.2614152488424715No Hit
AAAAGA2596190.2591948820065216No Hit
AATGCA2559390.2555208937168201No Hit
CAAAAA2537190.2533045203464024No Hit
AACAAA2528590.2524459252569613No Hit
AAATAA2522210.25180896750456194No Hit
TTTTTT2449330.2445328733047402No Hit
TAAAAA2346820.23429861951187891No Hit
AAAGAC2286250.2282515143296176No Hit
AATGAA2230050.2226406952567583No Hit
AAAACC2212340.2208725883923395No Hit
AAGGAA2199150.2195557431330688No Hit
GAAGCC2179730.2176169156171448No Hit
AAAAAT2173040.2169490085068703No Hit
ATAAAA2139360.21358651052868702No Hit
AATGAC2117070.21136115186082166No Hit
AAAATA1930610.19274561228207895No Hit
AAGGCC1800600.1797658509357723No Hit
NTNNNN1677690.16749492972144608No Hit
AATACC1589300.15867036926148112No Hit
AAAGAG1579840.15772591466309593No Hit
NCNNNN1573710.15711391607027336No Hit
AAAGGA1475750.14733391898170942No Hit
AGAGAA1467180.14647831899141756No Hit
AATACA1466110.14637149378842895No Hit
AAACAC1411520.1409214117032441No Hit
GAAGAA1409210.1406907890687547No Hit
AAGGCA1406150.14038528895553495No Hit
ACTGCC1332100.13299238588889387No Hit
NAGAAA1279170.12770803262329883No Hit
NAAGAA1269270.12671964990405848No Hit
AATTAA1265970.12639018899764504No Hit
CCCCCA1202310.12003458860301477No Hit
AGGAAA1196730.11947750016126112No Hit
NATAAA1188980.11870376621438107No Hit
AATTCC1186160.11842222689435504No Hit
AATAAC1177690.11757661056789387No Hit
GGAAAA1170100.11681885048314294No Hit
AAGGAC1167350.1165442997277984No Hit
AAACCA1161170.11592730930306049No Hit
AACCAA1150750.11488701153190048No Hit
ACCAAA1123250.1121415039784551No Hit
CCCCAA1121960.11201271471503893No Hit
CATGCC1119610.11177809861501722No Hit
NGAAAA1097620.109582690938644No Hit
NAAACA1092970.10911845057051596No Hit
AAACCC1089370.10875903867261039No Hit
AACACA1086530.10847550261981823No Hit
CCAAAA1072530.10707778968351875No Hit
ACAACA1072080.10703286319628055No Hit
AAGACA1069930.10681821442392028No Hit
NAAAAG1065190.10634498875834461No Hit
ACACAA1016310.10146497387789336No Hit
TTAAAA1007090.10054448007270184No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)