HYN2CBCX2_l01.1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	HYN2CBCX2_l01.1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	100163629
Sequences flagged as poor quality	0
Sequence length	51
%GC	46

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGA	25958902	25.91649509823571	Illumina Paired End PCR Primer 2 (100% over 32bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGC	1955916	1.9527207825107855	Illumina Paired End PCR Primer 2 (97% over 36bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGG	1181124	1.1791944958384046	Illumina Paired End PCR Primer 2 (96% over 33bp)
CAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCG	777138	0.7758684542070655	Illumina Paired End PCR Primer 2 (100% over 37bp)
CGGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCG	117152	0.11696061850953902	Illumina Paired End PCR Primer 2 (100% over 31bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTC	100970	0.10080505369868338	Illumina Paired End PCR Primer 2 (100% over 51bp)

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
CCGAGAT	3303260	37.955986	17
CGAGATC	3303795	37.893715	18
GCCGAGA	3431185	36.65397	16
GAGATCG	3430975	36.50949	19
TGCCGAG	3454900	36.41936	15
AGATCGG	3678160	34.03832	20
TCGGAAG	3830060	32.60515	23
AGCGGTT	3820990	32.519295	30
GCGGTTC	3810745	32.499416	31
ATCGGAA	3847125	32.48629	22
GAGCGGT	3839685	32.36635	29
GATCGGA	3871590	32.30649	21
AGAGCGG	3866930	32.204163	28
AAGAGCG	3882445	32.10755	27
GAAGAGC	3917750	31.875145	26
GAGACCG	119450	31.69214	45
CGGAAGA	3946175	31.674002	24
GGAAGAG	3954885	31.643417	25
CGAGACC	130715	29.093594	44
CCGAGAC	130805	29.038837	43