FastQCFastQC Report
Tue 14 Mar 2017
HYMN3BGXY_n01_9w-9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYMN3BGXY_n01_9w-9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15517174
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCC168370.10850558226646168No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG35450.043.30235715
CTCGTAT24900.038.51798642
TCGTATG29900.037.2699943
CACGTCT43000.035.7792614
TATGCCG34600.033.1052546
ACACGTC48150.032.31061613
CAGTCAC47700.031.09985727
AGTCACC50750.029.02677328
GTCTGAA56200.028.17362417
CGTATGC40350.027.3611544
CTTGTAA54200.027.305234
TTGTAAT58900.024.83357635
ATGCCGT46550.024.82920647
TGTAATC44200.024.27326836
GTATGCC47600.023.9911145
TAATCTC42800.023.93867138
GTCGCTA43050.023.88094127
CGCTATG43400.023.60878629
CACACGT65850.023.52098512
TTACCTC34500.023.39723415
Produced by FastQC (version 0.11.2)