FastQCFastQC Report
Tue 14 Mar 2017
HYMN3BGXY_n01_9w-4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYMN3BGXY_n01_9w-4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15154612
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCC199160.1314187390610858No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG34950.041.9487815
CTCGTAT29950.041.23792342
TCGTATG33200.038.7604243
AGTCACG35700.037.3015428
CACGGCT36400.037.24543831
TATGCCG36750.034.9230346
CACGTCT43500.034.1001914
ACACGTC46450.032.15731413
CAGTCAC44350.030.64857727
TTTTTCC86700.028.7071781
ATGCCGT46500.028.0463747
GTATGCC46550.027.49615345
TGTTACC37250.027.13432113
GTCTGAA54950.026.80591217
GCTACAT50700.026.67262535
CGTATGC48550.026.50566744
TTACCTC38650.025.97293915
GTCACGG54100.025.44404629
TTTTCCA100700.025.3870472
TAGTTGT42550.024.9700939
Produced by FastQC (version 0.11.2)