FastQCFastQC Report
Tue 14 Mar 2017
HYMN3BGXY_n01_9w-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYMN3BGXY_n01_9w-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12499055
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCC184140.14732313762920476No Hit
GTAAAATCAAAAGACGATGAACAAACTGGGAACACACGAAACAAGGTGGGAGACATCATCGAATCAGAGATGAAC130760.10461590896271758No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG29000.044.13593343
AGTCACG28800.043.8420828
CTCGTAT26750.043.59120642
ACGTCTG32200.042.10283715
TATGCCG32300.039.19975746
GTCACGA32750.038.55425329
CACGTCT37450.036.56888214
CGTATGC36300.034.88036744
CAGTCAC38600.033.247727
ACACGTC42100.032.85755513
GTATGCC40600.031.44094345
ATGCCGT42050.030.43918247
TGTTACC35550.028.72268913
TCACGAT44850.028.4601130
TTTTTCC81900.028.4080181
CGCTATG31300.028.21617529
CACGATC46200.027.70271131
TCTAGTT41900.027.3341587
GTCTGAA50600.026.99713317
TTTTCCA89700.026.3470442
Produced by FastQC (version 0.11.2)