FastQCFastQC Report
Tue 14 Mar 2017
HYMN3BGXY_n01_9w-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYMN3BGXY_n01_9w-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16030513
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCCTGTGGTAACT169190.1055424739058569No Hit
CTTAAACCTATAACCATCTTTCGGCTAACCTAGCCTCCTCCGTCCCTCCGTACCAACAAGGGGTAGTACAGGAAT163890.10223627902612974No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG37750.046.603915
CTCGTAT33300.045.06757744
TCGTATG36650.044.80751445
TATGCCG38000.043.1260948
CACGTCT43500.040.60196714
GTCACAG45300.037.16429529
AGTCACA45550.037.0362928
ACACGTC48650.036.87104813
GTATGCC45950.036.64040847
CAGTCAC46400.036.28347427
ATGCCGT48450.034.10996649
CACAGTC49000.033.86337731
CGTATGC49800.033.2533346
ACAGTCA50750.032.9674732
GTCTGAA56400.031.2543817
GCCGTCT58800.027.40265551
CTTAAAC44950.027.3396471
GTCAACA61050.027.23612435
CGCTATG52300.026.91303829
TCACAGT62500.026.71480630
Produced by FastQC (version 0.11.2)