FastQCFastQC Report
Tue 14 Mar 2017
HYMN3BGXY_n01_9w-18.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYMN3BGXY_n01_9w-18.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15798755
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCC178170.11277470914638527No Hit
CCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTATAAGCAACATCCGCCGATCCCTGGTCG160260.10143837283380873No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG39050.039.84104515
AGTCACT40250.036.1697228
ACACGTC44550.035.38688313
CTTATCG18350.034.96748737
CACGTCT46750.033.35267314
CAGTCAC46650.031.79924227
TGTTACC35700.028.40880413
GTCTGAA55400.028.39406417
TTACCTC36850.027.61593215
GTCGCTA49750.026.97469127
CGCTATG50300.026.67957129
TTTTTCC83200.026.2607561
TAGTTGT42800.024.1794959
CTAATGC41400.023.83167821
CTTAAAC40400.023.7514551
TTTTCCA96750.023.6421662
TCTAGTT49800.023.621037
TCTAATG42050.023.54518520
CACACGT67550.023.28698712
AGCACAC70000.023.0139610
Produced by FastQC (version 0.11.2)