FastQCFastQC Report
Tue 14 Mar 2017
HYMN3BGXY_n01_9w-13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYMN3BGXY_n01_9w-13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15115367
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG28700.041.46831515
CACGTCT36350.033.12064414
ACACGTC39150.031.19243213
AGTCACA39200.029.57037528
GTCACAT41800.026.98800729
CAGTCAC42950.026.42621427
GTCGCTA44700.025.854727
CGCTATG45800.025.0829229
GTCTGAA51650.023.64328617
CTTAAAC38100.022.5611461
TTACCTC28500.021.18226615
GCTATGA56750.020.48615630
CTATAAG34800.020.0260846
ATCGGAA59300.020.0150972
GGTAACT57450.019.9974769
ACATGCG41050.019.6672534
CACACGT62650.019.49215912
ATGTCAG58000.019.33018734
AATCGCG12150.019.30770141
CACATGT61450.019.14320231
Produced by FastQC (version 0.11.2)