FastQCFastQC Report
Tue 14 Mar 2017
HYMN3BGXY_n01_9w-11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYMN3BGXY_n01_9w-11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13654547
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCC166800.12215711000884907No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCT19300.048.43980439
ACGTCTG29450.042.0520215
CTCGTAT21700.038.79176744
TTCCGTA32150.035.19492736
CACGTCT35450.034.837314
TCGTATG23700.034.35354645
AGTCACA34000.033.585928
ACACGTC37700.033.3986713
TATGCCG25850.033.2325948
CAGTCAC35600.032.1733327
TCCGTAT35700.031.11536437
TATCTCG29550.030.70389441
GTCACAG37950.030.1811529
CCGTATC35150.030.13019238
TGTTACC32900.028.20551513
GTCTGAA44800.027.95167717
GTATGCC33950.026.62454247
AGTTCCG45100.025.2420534
CGCTATG38000.025.23891629
TCACAGT45800.025.0831430
Produced by FastQC (version 0.11.2)