FastQCFastQC Report
Tue 14 Mar 2017
HYMN3BGXY_n01_9s-9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYMN3BGXY_n01_9s-9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15344820
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCC174880.11396679791616975No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG31200.043.89514515
TCGTATG29000.043.6590343
CTCGTAT25900.042.75712642
TATGCCG33100.039.08543846
ACTGACC34150.037.7811932
CACGTCT37000.037.107414
AGTCACT35200.036.9500228
ACACGTC42400.033.113713
CAGTCAC40050.032.21689227
CGTATGC40850.031.24773444
GTATGCC44600.029.08461245
ATGCCGT45100.028.9920347
CGCTATG38200.027.63592529
GTCGCTA39400.026.9692627
CTGACCA49800.026.25446533
TTTTTCC78300.025.9221341
TGTTACC34200.025.62045113
GTCTGAA54950.025.04865317
TTACCTC38150.022.87739615
GGTAACT45800.022.67371269
Produced by FastQC (version 0.11.2)