FastQCFastQC Report
Tue 14 Mar 2017
HYMN3BGXY_n01_9s-4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYMN3BGXY_n01_9s-4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13266696
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCC186060.14024592106429515No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTAGG26850.044.1972732
ACGTCTG31950.040.59709515
AGTCACT33200.035.84953328
GTCACTT33550.035.5785129
CTCGTAT20050.035.10143742
TTAGGCA35000.034.1028734
ACACGTC39100.033.7024413
CACGTCT39000.033.16981514
CAGTCAC37200.032.45831727
TCGTATG20150.031.33190243
TATGCCG21300.029.15502746
TTTTTCC78300.029.052811
GTCTGAA48750.027.1016417
TGTTACC35500.026.81979413
TTTTCCA91150.025.1709862
TTACCTC38100.024.9897615
TCTAGTT44900.024.354797
CACACGT53850.024.15073412
TAGTTGT40950.023.6713949
TCTAATG39250.023.64323820
Produced by FastQC (version 0.11.2)