FastQCFastQC Report
Tue 14 Mar 2017
HYMN3BGXY_n01_9s-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYMN3BGXY_n01_9s-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16113874
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAAAATCAAAAGACGATGAACAAACTGGGAACACACGAAACAAGGTGGGAGACATCATCGAATCAGAGATGAAC178860.11099751679825721No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT34900.037.7611242
ACGTCTG40050.037.5544415
TCGTATG39200.036.6999143
TATGCCG42950.033.73721746
CGTATGC43650.032.56357244
CACGTCT49650.030.84899314
AGTCACA52600.027.80893328
TCACGAT54050.026.87067235
ACACGTC57550.026.79403513
CAGTCAC54750.026.59070627
ATCACGA56050.026.15797234
GTATGCC57150.025.17322745
ATGCCGT58600.024.90435447
CACACGT63100.024.6013612
CGTCTGA61300.024.59217316
ATCGGAA61500.023.786622
GTCACAT63900.022.78304729
CCAGTCA67100.022.10803826
TCGGAAG67850.021.5090073
GTCTGAA72650.021.41487317
Produced by FastQC (version 0.11.2)