FastQCFastQC Report
Tue 14 Mar 2017
HYMN3BGXY_n01_9s-30.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYMN3BGXY_n01_9s-30.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17237487
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTATAAGCAACATCCGCCGATCCCTGGTCG224960.1305062623107417No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG33850.038.72653215
GTCACAC32550.037.2017829
CACGTCT38100.034.8590614
AGTCACA35500.034.01328328
CAGTCAC38750.031.42773827
TCACACT40850.030.31795530
ACACGTC45650.029.471613
CTTAAAC44350.026.9321541
ACCTATA45400.026.3695456
CACACTT46700.026.29802131
CTATAAG67350.026.07303246
CGCTATG44550.025.94207229
AATCGCG12550.025.83882939
GTCTGAA53150.024.59883717
TATAAGC71100.024.50385547
GTCGCTA47800.024.1783727
TAAACCT50900.023.7913073
ACATCCG72950.023.65044255
TATAACC49750.023.5754189
TGTTACC29000.023.55304513
Produced by FastQC (version 0.11.2)