FastQCFastQC Report
Tue 14 Mar 2017
HYMN3BGXY_n01_9s-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYMN3BGXY_n01_9s-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15267883
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG26850.040.59988415
GTCGTAT10250.037.69656842
GTCACAC29800.034.49886329
CACGTCT36700.029.79718214
ACACGTC38050.029.46519513
CAGTCAC35900.028.7331327
AGTCACA36450.028.20492628
TCGTATG19350.028.17016843
CACAGTG36550.027.84313233
CTTAAAC39850.027.1139341
TATGCCG23450.026.04101846
ACACAGT40300.025.2522732
ACCTATA41000.025.2445586
CACACAG42250.024.57668131
CGCTATG36350.024.10649529
TGTTACC30900.023.4445213
TTAAACC48200.022.7623752
GTCGCTA39250.022.67703227
GTCTGAA49200.022.43688817
TAAACCT48250.022.2379553
Produced by FastQC (version 0.11.2)